| 6-苄基腺嘌呤对水稻颖花分化影响机制的研究 |
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| 引用本文： | 黄静静,王绍华,李刚华,刘正辉,丁艳锋.6-苄基腺嘌呤对水稻颖花分化影响机制的研究[J].南京农业大学学报,2009,32(3). |
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| 作者姓名： | 黄静静 王绍华 李刚华 刘正辉 丁艳锋 |
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| 作者单位： | 南京农业大学农业部作物生长调控重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 以常规籼稻品种扬稻6号为材料,在盆栽条件下,于生长锥伸长期外喷6-苄基腺嘌呤(6-BA)后,定期取样,测量穗部细胞分裂素(cytokinins,CKs)含量,同时采用RT-PCR检测水稻穗分化过程中细胞分裂素氧化酶基因(OsCKX2)的表达模式,考察水稻穗部性状.结果发现:6-BA处理促进水稻穗长发育并显著提高每穗枝梗和颖花数,分化枝梗数和总颖花数分别为60和255.4,比对照分别提高了10%和30.5%.穗部细胞分裂素含量的增加,刺激了更多颖花分化.6-BA 处理的水稻穗部OsCKX2表达时间提前,但表达量下降.认为:水稻穗分化始期喷施6-BA可降低穗部OsCKX2的表达强度,提高穗部细胞分裂素含量,对枝梗和颖花分化产生促进作用,为形成大穗提供了先决条件.
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| 关 键 词： | 水稻 颖花分化 6-苄基腺嘌呤(6-BA) 水稻细胞分裂素氧化酶基因(OsCKX2) |
The study of mechanism of 6-benzyl adenine influencing spikelet differentiation of rice |
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| HUANG Jing-jing,WANG Shao-hua,LI Gang-hua,LIU Zheng-hui,DING Yan-feng.The study of mechanism of 6-benzyl adenine influencing spikelet differentiation of rice[J].Journal of Nanjing Agricultural University,2009,32(3). |
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| Authors: | HUANG Jing-jing WANG Shao-hua LI Gang-hua LIU Zheng-hui DING Yan-feng |
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| Institution: | Key Laboratory of Crop Growth Regulation;Ministry of Agriculture;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | This study employed pot experiment and used an indica rice cultivar Yangdao 6 to investigate the character of panicles,the level of cytokinin in panicle and the expression pattern of Oryza sativa cytokinin oxidase/dehydrogenase gene(OsCKX2)during rice panicle differentiation process by performing 6-BA treatment at the time of bud differentiation stage.Samplings were made regularly.6-BA had beneficial effect on panicle length and the number of total branches,spikelets,the number of branches and spiklets were... |
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