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虾池沉积物中3类主要细菌的垂直分布特征
引用本文:李秋芬,陈碧鹃,曲克明,辛福言,李健,赵法箴,袁有宪.虾池沉积物中3类主要细菌的垂直分布特征[J].中国水产科学,2002,9(4):367-370.
作者姓名:李秋芬  陈碧鹃  曲克明  辛福言  李健  赵法箴  袁有宪
作者单位:中国水产科学研究院,黄海水产研究所,山东,青岛,266071
基金项目:国家“八六三”计划资助项目 (863 -891-0 2 -0 7),国家自然科学基金资助项目 (3 9670 5 81),农业部重点课题资助项目 (渔95 -B -96-0 6-0 7)
摘    要:采用平板涂布法和MPN法测定了虾池底质下 0到 30cm深度范围内 3类主要细菌类群的垂直分布情况。结果表明 ,底泥中细菌主要集中于 0到 5cm的表层范围内 ,随深度增加 ,数量急剧减少 ,至 30cm深处所测到菌量已很少。底泥中的总菌量随养殖时间推移 ,逐渐增加 ,到养殖中后期 ,表层菌量增加至 10 6CFU/g ,表层以下 10~2 0cm的总异养菌量和硝酸盐还原菌数量也增加至 10 5CFU/ g以上。弧菌仍集中于表层。细菌的垂直分布主要受各层有机物和溶解氧含量的影响

关 键 词:对虾养殖  沉积物  总异养菌  弧菌  硝酸盐还原菌  垂直分布
文章编号:1005-8737(2002)04-0367-04
修稿时间:2001年8月7日

Vertical distribution of three main bacteria in sediment of shrimp ponds
LI Qiu fen,CHEN Bi juan,QU Ke ming,XIN Fu yan,LI Jian,ZHAO Fa zhen,YUAN You xian.Vertical distribution of three main bacteria in sediment of shrimp ponds[J].Journal of Fishery Sciences of China,2002,9(4):367-370.
Authors:LI Qiu fen  CHEN Bi juan  QU Ke ming  XIN Fu yan  LI Jian  ZHAO Fa zhen  YUAN You xian
Abstract:The shrimp ponds were stocked with 50 000 juvenile shrimps with body length 3.5 cm. The bottom mud was sampled before the stock, during the culture (the early stage, middle stage and post stage of the culture) and at the end of the culture. Spread plant method and multiple possible number (MPN) method were used to determine the distribution of the bacteria. The results show that most of the bacteria in the sediments focus on the surface layer within 0-5 cm in depth and the bacterium number drops rapidly with the increase of depth under the bottom, and at the depth of 30 cm, almost no bacteria can be detected. The total number of bacteria increases with the cultural time, and up to the final stage of the culture season, the heterotrophic bacteria and nitrate reducing bacteria on the surface layer of the sediments increase to 10 6 CFU/g and those of under layer within the depth of 10-20 cm also get to over 10 5 CFU/g, while the vibrio bacteria mostly distribute on the surface layer. The vertical distribution of bacteria numbers correlates with the distribution of organic matter and dissolved oxygen.
Keywords:shrimp culture  sediment  heterotrophic bacteria  vibrio bacteria  nitrate  reducing bacteria  vertical distribution
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