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| 不同耐寒甘蔗品种的SSR标记分析 | |
| 引用本文： | 廖诗童,贤武,周会,梁强,桂意云,覃丽翻,杨荣仲.不同耐寒甘蔗品种的SSR标记分析[J].热带作物学报,2012,33(12):2130-2137. |
| 作者姓名： | 廖诗童 贤武 周会 梁强 桂意云 覃丽翻 杨荣仲 |
| 作者单位： | 1. 中国农业科学院甘蔗研究中心,广西南宁530007;广西大学,广西南宁530004;广西农业科学院甘蔗研究所,广西南宁530007;农业部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007 2. 中国农业科学院甘蔗研究中心,广西南宁530007;农业部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007 3. 中国农业科学院甘蔗研究中心,广西南宁530007;广西大学,广西南宁530004;农业部广西甘蔗生物技术与遗传改良重点实验室,广西南宁530007 |
| 基金项目： | 现代农业甘蔗产业技术体系（CARS：20-1-3）。 |
| 摘 要： | 采用197对EST-SSR引物和67对gSSR引物对35个不同耐寒甘蔗品种进行标记,并筛选出36对多态性较好的EST-SSR引物和17对gSSR引物进行遗传多样性分析.结果表明:17对gSSR引物共扩增出87条谱带,平均每对引物扩增出5.1条谱带,PIC值在0.53～0.93,平均值为0.84,引物的DP值在0.54～0.96,平均值为0.87.36对EST-SSR引物共扩增出162条谱带,平均每对引物扩增出4.5条谱带,PIC值在0.43 ～0.93,平均值为0.79,引物的DP值在0.44～0.96,平均值为0.81.品种间的gSSR遗传相似系数在0.413 8～0.804 6,平均值为0.625 1;品种间的EST-SSR遗传相似系数在0.5145～0.797 1,平均值为0.665 1;因此,本研究中gSSR标记的效果要好于EST-SSR.聚类分析结果表明,35份甘蔗品种可分为7个类群;然而,聚类分析结果并不能反映供试材料的地域特性.耐寒性相对较好的品种分散于5个类群中,说明聚类分析结果并不能把耐寒性好的品种聚成一类;而两三个耐寒性较差的品种能够聚在一起,说明SSR标记对耐寒性较差的品种的聚类起到较好的作用.以上结果为SSR标记在甘蔗耐寒性研究上的应用提供起到一定的参考作用. |
| 关 键 词： | 甘蔗 SSR EST-SSR 耐寒性 聚类分析 |
Assessment of Cold Tolerance in Different Sugarcane Varieties Using SSR Markers |
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| LIAO Shitong,XIAN Wu,ZHOU Hui,LIANG Qiang,GUI Yiyun,Qin Lifan and YANG Rongzhong.Assessment of Cold Tolerance in Different Sugarcane Varieties Using SSR Markers[J].Chinese Journal of Tropical Crops,2012,33(12):2130-2137. | |
| Authors: | LIAO Shitong XIAN Wu ZHOU Hui LIANG Qiang GUI Yiyun Qin Lifan and YANG Rongzhong |
| Institution: | 1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences 2 Guangxi University 3 Guangxi Sugarcane Research Institute 4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences 2 Guangxi University 4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China;1 Sugarcane Research Center, Chinese Academy of Agricultural Sciences 2 Guangxi University 3 Guangxi Sugarcane Research Institute 4 Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi) Ministiv of Agriculture P R China |
| Abstract: | Genomic DNAs of 35 sugarcane varieties were marked by using 197 pairs of EST-SSR primers and 67 pairs of SSR primers, and the genetic diversity among the 35 sugarcane varieties was assessed using 36 pairs of EST-SSR polymorphic primers and 17 pairs of SSR polymorphic primers screened out from the above primers. The results indicated that 87 SSR bands were obtained, with a mean of 5.1 bands per primer. The PIC value was ranged from 0.53 to 0.93 with a mean value of 0.84. The DP value was ranged from 0.54 to 0.96 with a mean value of 0.87. And 162 EST-SSR bands were obtained, about 4.5 bands amplified by each primer. The PIC value was ranged from 0.43 to 0.93 with an average of 0.81. The DP value was ranged from 0.44 to 0.96 with an average of 0.81. The SSR genetic similarity coefficients of the sugarcane cultivars was ranged from 0.413 8 to 0.804 6, with the average of 0.625 1. And the EST-SSR genetic similarity coefficients of the sugarcane cultivars was ranged from 0.514 5 to 0.797 1, with the average of 0.665 1. The results showed that the effect of gSSR was better than that of EST-SSR. According to the results of clustering analysis, the 35 sugarcane varieties could be classified into 7 groups, but it could not reflect the characteristic of the zone of the tested sugarcane varieties. Some of the good cold tolerance varieties did not go to the same group, indicating that the good cold tolerance varieties could not be classified well by clustering analysis, but a few cold sensitive varieties could be classified into the same subgroup. It is concluded that the SSR markers were good for clustering the cold sensitive sugarcane varieties, which could be referred for research on cold resistance in sugarcane. |
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