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| 离子对色谱法测定茶叶中的茶氨酸 | |
| 引用本文： | 龚雨顺,黄建安,崔湘兴,李勤,李银花,陈金华.离子对色谱法测定茶叶中的茶氨酸[J].茶叶科学,2008,28(2):89-92. |
| 作者姓名： | 龚雨顺 黄建安 崔湘兴 李勤 李银花 陈金华 |
| 作者单位： | 1. 湖南农业大学茶学教育部重点实验室,湖南,长沙,4101282;湖南农业大学药用植物资源系,湖南,长沙,410128 2. 湖南农业大学茶学教育部重点实验室,湖南,长沙,4101282 3. 湖南农业大学国际学院,湖南,长沙,410128 |
| 基金项目： | 2004BA542C茶叶和辣椒功能成分高效提制技术研究与开发科技部十五攻关项目 |
| 摘 要： | 通过添加十二烷基磺酸钠(SDS)为离子对试剂,以乙腈-水(磷酸)为流动相,在200nm的检测波长条件下,建立了一种快速测定茶叶中茶氨酸的分析方法。茶氨酸的保留时间为6.27min;在0.04~20μg范围内,茶氨酸峰面积与进样量之间有良好的线性关系,回归方程为:Y=1.51466×106X+3.28706×105(r2=0.99901);当S/N=3时,最低检测浓度为1.15ng。所建方法快速,简便,准确,可用于茶叶中茶氨酸含量测定。 |
| 关 键 词： | 茶氨酸 离子对色谱 茶叶 |
| 文章编号： | 1000-369X(2008)02-089-04 |
| 修稿时间： | 2007年9月24日 |
Determination of Theanine in Tea by Paired Ion Chromatography |
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| GONG Yu-shun,HUANG Jian-an,CUI Xiang-xing,LI Qing,LI Yin-hua,CHEN Jin-hua.Determination of Theanine in Tea by Paired Ion Chromatography[J].Journal of Tea Science,2008,28(2):89-92. | |
| Authors: | GONG Yu-shun HUANG Jian-an CUI Xiang-xing LI Qing LI Yin-hua CHEN Jin-hua |
| Abstract: | A paired ion chromatographic method was developed for rapid determination of theanine in tea. SDS was added as a paired ion reagent, mobile phase was a mixture of acetonitrile and phosphoric acid aqueous solution, detection wavelength was set at 200 nm. The retention time of Theanine was 6.27 min. The calibration of theanine was in good linearity in the range of 0.04~20 μg with the regression equation Y=1.51466×106X+3.28706×105(r2=0.99901). The detection limits was 1.15 ng(S/N=3). This method is rapid, simple, and accurate for the analysis of theanine in tea. |
| Keywords: | theanine paired Ion Chromatography tea |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |