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| 柳桉组培快繁技术 | |
| 引用本文： | 吴美华,林文革,兰贺胜,杨宗武,李海花,陈水洲.柳桉组培快繁技术[J].福建林业科技,2006,33(4):159-162. |
| 作者姓名： | 吴美华 林文革 兰贺胜 杨宗武 李海花 陈水洲 |
| 作者单位： | 1. 福建省永安林业(集团)股份有限公司种苗中心,福建,永安,366000 2. 福建省林木种苗总站,福建,福州,350003 3. 福建省林业科学研究院,福建,福州,350012 |
| 基金项目： | 福建省林木种苗科技攻关项目 |
| 摘 要： | 以3年生柳桉优树环割促萌枝条为外植体,以MS为基本培养基,通过激素种类与浓度、大量元素配比等试验,探讨了柳桉腋芽诱导、继代增殖、生根培养基配方。结果表明:外植体宜采用中等幼嫩的茎段,用75%酒精消毒30 s、0.1%升汞溶液消毒4 min效果最佳;在3/4 MS+0.3 mg.L-16-BA+0.2 mg.L-1NAA培养基上进行侧芽诱导,柳桉外植体出芽率最高;在改良MS+0.2 mg.L-16-BA+0.2 mg.L-1NAA继代培养基上,柳桉增殖效果最好,其芽体健壮,生长正常,增殖率3倍以上;应用1/2MS培养基附加ABT 1#0.5 mg.L-1和IBA 0.1 mg.L-1,其生根率可达95%以上,生根3~4条,苗木生长健壮,移栽成活率可达92%以上。这些配方已成功地应用于柳桉优良无性系苗木的工厂化生产,单个超净台月苗木生产能力达7万株以上。 |
| 关 键 词： | 柳桉 组织培养 快繁技术 育苗 |
| 文章编号： | 1002-7351（2006）04-0159-04 |
| 收稿时间： | 2006-06-07 |
| 修稿时间： | 2006-06-07 |
Micropropagation of Eucalyptus saligna |
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| WU Mei-hua,LIN Wen-ge,LAN He-sheng,YANG Zong-wu,LI Hai-hua,CHEN Shui-zhou.Micropropagation of Eucalyptus saligna[J].Journal of Fujian Forestry Science and Technology,2006,33(4):159-162. | |
| Authors: | WU Mei-hua LIN Wen-ge LAN He-sheng YANG Zong-wu LI Hai-hua CHEN Shui-zhou |
| Abstract: | A tissue culture protocol by shoot proliferation is developed for Eucalyptus saligna through adjusting the plant growth regulators and ratio of macro-elements using sprouts collected from stubs of three-year-old plus trees as explants.The main results are as follows: 1.Half lignified shoots were most suitable for explants.Dipping them in solution of 75% alcohol for 30 seconds and then in 0.1% HgCl_2 for 4 minutes are the best way for their disinfection;2.3/4 MS supplemented with 0.3 mg·L~(-1) 6-BA,0.2 mg·L~(-1) NAA and 20 g·L~(-1) sugar is crucial for shoot germination and proliferation,while amended MS supplemented with 0.2 mg·L~(-1) 6-BA,0.2 mg·L~(-1) NAA and 30 g·L~(-1) sugar for clump shoot induction;3.1/2 MS supplemented with 0.5 mg·L~(-1) ABT-1 and 0.1 mg·L~(-1) IBA is suitable for rooting,with rooting rate up to 95%;and 4.The survival rate of out-planting in nursery is more than 92%.The protocol has been successfully used in large-scale clonal production of the seedlings. |
| Keywords: | Euaclyptus saligna tissue culture micropropagation raise seedlings |
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