| 根癌农杆菌介导Cry1Ac基因转化‘雪青’梨获得转基因植株 |
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| 引用本文： | 汤绍虎,孙敏,廖志华,周启贵,李道高.根癌农杆菌介导Cry1Ac基因转化‘雪青’梨获得转基因植株[J].园艺学报,2007,34(1):59-62. |
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| 作者姓名： | 汤绍虎 孙敏 廖志华 周启贵 李道高 |
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| 作者单位： | ( 西南大学生命科学学院, 重庆400715; 西南大学园艺园林学院, 重庆400716) |
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| 摘 要： | 以‘雪青’梨叶片愈伤组织为外植体, 经根癌农杆菌介导将CaMV35S启动子调控下的Cry1Ac基因导入‘雪青’梨。698块愈伤组织和231个Kanr芽与携带表达载体质粒pBX203的根癌农杆菌菌株LBA4404共培养3 d后, 转入含50 mg·L-1 Kan的筛选培养基培养30 d, 15 d转接1次。结果表明,在MS + 5 mg·L -1 6-BA + 0.1 mg·L-1 IAA筛选培养基中, Kanr芽率为34.24% , 在1/2MS + 2 mg·L-1 IBA+ 0.5 mg·L-1 6-BA + 500 mg·L-1AC筛选培养基中, 15.58% Kanr芽生根。共获得再生植株32株, 经PCR和Southern-blot分析证明, 其中4株的基因组已成功导入和整合Cry1Ac基因。
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| 关 键 词： | 梨 根癌农杆菌 Cry1Ac基因 遗传转化 |
| 文章编号： | 0513-353X（2007）01-0059-04 |
| 收稿时间： | 2006-06-27 |
| 修稿时间： | 2006-06-272006-10-08 |
Obtainment of Transgenic 'Xueqing' Pear Plants with a Synthetic Cry1Ac Gene Mediated by Agrobacterium tumefaciens |
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| TANG Shao-hu,SUN Min,LIAO Zhi-hua,ZHOU Qi-gui,LI Dao-gao.Obtainment of Transgenic ''''Xueqing'''' Pear Plants with a Synthetic Cry1Ac Gene Mediated by Agrobacterium tumefaciens[J].Acta Horticulturae Sinica,2007,34(1):59-62. |
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| Authors: | TANG Shao-hu SUN Min LIAO Zhi-hua ZHOU Qi-gui LI Dao-gao |
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| Institution: | ( School of Life Science, Southwest University, Chongqing 400715, China; College of Horticulture and Gardening, SouthwestUniversity, Chongqing 400716, China) |
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| Abstract: | By using calli from leaves of Xueqing pear ( Pyrus sp. ) as explants, the Cry1Ac gene regulated by CaMV35S promoter was introduced into Xueqing pear through Ag robacterium-mediated transformation.After co-culturing 698 calli and 231 Kanr buds, respectively, with A. tum efaciens strain LBA4404 harboring aplasmid pBX203 for 3 days and transferred to the selective medium containing 50 mg·L-1 kanamycin for 30days at a 15-day interval, 34.24% kanamycin2resistant (Kanr ) buds ( adventitious shoots) regenerated on MS+ 5 mg·L-1 6-BA + 0.1 mg·L-1 IAA and 15.58% of Kanr buds rooted on 1/2 MS + 2 mg·L-1 IBA + 0.5mg·L-1 6-BA + 500 mg·L-1 AC. A total of 32 plants were obtained, but only 4 p lants were confirmed byPCR and Southern-blot analysis that the Cry1Ac gene was transferred and integrated into the genome ofXueqing pear. |
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| Keywords: | Pear Agrobacterium tumefaciens Cry1Ac gene Genetic transformation |
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