| 双浊点萃取-毛细管电泳法分离测定井水中痕量硝基苯酚位置异构体 |
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| 引用本文： | 程佳,侯文欣,程晓昆,杨更亮.双浊点萃取-毛细管电泳法分离测定井水中痕量硝基苯酚位置异构体[J].安徽农业科学,2011,39(12):7338-7339,7341. |
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| 作者姓名： | 程佳 侯文欣 程晓昆 杨更亮 |
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| 作者单位： | 河北大学药学院,河北省药物质量分析控制重点实验室,河北保定,071002 |
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| 摘 要： | 目的]为测定井水中痕量硝基苯酚位置异构体提供参考。方法]采用双浊点萃取进行预处理,结合毛细管电泳技术对萃取物进行检测。结果]选择浓度为0.5%Triton X-114为表面活性,pH 1.5,添加浓度0.020 g/ml NaCl,同时添加浓度0.2 mol/L NaOH溶液100μl,40℃时加热20min条件下双浊点萃取-毛细管电泳法分离测定井水中痕量硝基苯酚位置异构体,邻硝基苯酚、间硝基苯酚在0.000 2～0.005 0 mg/ml浓度范围内均呈现良好的线性关系,方法的加标回收率可达97%,相对标准偏差均小于5%。结论]该方法消除了富集相中表面活性剂对毛细管电泳分离的影响,分析结果准确,且对环境友好。
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| 关 键 词： | 双浊点萃取 毛细管电泳 邻硝基苯酚 间硝基苯酚 |
Determination and Isolation of the Nitrophenol Isomers in Well Water with Dual-Cloud Point Extraction and Capillary Electrophoresis |
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| CHENG Jia et al.Determination and Isolation of the Nitrophenol Isomers in Well Water with Dual-Cloud Point Extraction and Capillary Electrophoresis[J].Journal of Anhui Agricultural Sciences,2011,39(12):7338-7339,7341. |
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| Authors: | CHENG Jia |
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| Institution: | CHENG Jia et al(Key Laboratory for Drug Quality Analysis and Control of Hebei Province,College of Pharmacy,Hebei University,Baoding,Hebei,071002) |
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| Abstract: | Objective] The reference for the determinate of the nitrophenol isomers in well water provided through the research.Method] The material from well water was pretreatment with Dual-Cloud Point Extraction and measured with Capillary Electrophoresis.Results] In the optimum condition,which concentration of the surfactant Triton X-114 was 0.5%,the pH was 1.5,the adding amount of NaCl was 0.020 g/ml,meanwhile,the adding amount of 0.2 mol/L NaOH was 100 μl,the nitrophenol isomers in well water were tested with the method of Dual-Cloud Point Extraction and Capillary Electrophoresis at the temperature of 40 ℃ for 20 minutes.There was good linearity between O-nitrophenol and M-nitrophenol in the range of 0.000 2-0.005 0 mg/mL,the recovery of the technique reached 97% and the relative standard deviation was less than 5%.Conclusion] The result from the technique was accurate and environment-friendship because the effect of the surfactant in the accumulation phase on the isolation of capillary electrophoresis was eliminated. |
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| Keywords: | Dual-Cloud Point Extraction Capillary Electrophoresis O-nitrophenol m-nitrophenol |
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