Plaque assay of bovine rotavirus |
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| Authors: | G Butchaiah E Lund |
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| Institution: | Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Copenhagen V, Denmark |
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| Abstract: | Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.Key words: bovine rotavirus, plaque assay, trypsin, diethyl-aminoethy1-dextran |
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