| 羊泰勒虫不同PCR检测方法的比较 |
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| 引用本文： | 张守发,田万年,王中光,其木格,贾立军,薛书江.羊泰勒虫不同PCR检测方法的比较[J].畜牧与兽医,2008,40(12). |
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| 作者姓名： | 张守发 田万年 王中光 其木格 贾立军 薛书江 |
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| 作者单位： | 延边大学农学院动物医学系,吉林,龙井,133400 |
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| 摘 要： | 为寻求快速、有效检测羊泰勒虫的PCR方法,本试验建立了检测羊泰勒虫18SrRNA基因和表面蛋白基因的两种常规PCR方法和一种检测18SrRNA基因的半套式PCR方法,并从其敏感性和临床样本检出率等方面进行了比较。结果显示:上述方法检测羊泰勒虫基因组DNA的最小检测量分别为1.6fg/μL、16fg/μL和0.016fg/μL;检测临床样本阳性检出率分别为31.37%(16/51)、17.64%(9/51)和45.10%(23/51)。3种方法中,检测18SrRNA基因的半套式PCR方法敏感性和临床样本检出率最高,其次为检测18SrRNA基因的常规PCR方法,最后为检测表面蛋白基因的常规PCR方法。结果说明,所建立的半套式PCR方法是一种较好的羊泰勒虫检测方法。
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| 关 键 词： | 羊泰勒虫 PCR 半套式PCR 18S rRNA基因 表面蛋白基因 |
Comparison of different PCR methods for the detection of Theileria |
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| ZHANG Shou-fa,TIAN Wan-nian,WANG Zhong-guang,QI Mu-ge,JIA Li-jun,XUE Shu-jiang.Comparison of different PCR methods for the detection of Theileria[J].Animal Husbandry & Veterinary Medicine,2008,40(12). |
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| Authors: | ZHANG Shou-fa TIAN Wan-nian WANG Zhong-guang QI Mu-ge JIA Li-jun XUE Shu-jiang |
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| Abstract: | To seek a rapid and valid PCR method for the detection of Theileria,we established two kinds of routine PCR for 18S rRNA gene and surface protein gene,respectively,and a semi-nested PCR for 18S rRNA gene.Their sensitivities and positive rates of the samples were detected and compared.The results showed that using the semi-nested PCR and the routine PCR to detect 18S rRNA and the routine PCR to detect surface protein gene,the minimum detection amounts were estimated 0.016 fg/μL,1.6 fg/μL and 16 fg/μL,respectively;the positive rates were 45.10%(23/51),31.37%(16/51)and 17.64%(9/51),respectively.It suggested that the semi-nested PCR had a higher sensitivity for Theileria than the other two routine PCR,and could be used to detect Theileria in clinical samples. |
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| Keywords: | PCR 18S |
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