| 1株猪源肠道益生菌的分离鉴定及其生物学特性研究 |
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| 引用本文： | 高书锋,刘惠知,周映华,缪东,吴胜莲,周小玲.1株猪源肠道益生菌的分离鉴定及其生物学特性研究[J].畜牧与兽医,2012,44(1):19-23. |
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| 作者姓名： | 高书锋 刘惠知 周映华 缪东 吴胜莲 周小玲 |
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| 作者单位： | 湖南省微生物研究所,湖南长沙,410009 |
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| 基金项目： | 长沙市科技攻关资金专项 |
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| 摘 要： | 从健康猪肠道中分离出1株对仔猪大肠杆菌病和副伤寒病病原有较强拮抗作用的菌株,命名为W-02,对其进行菌落形态、生理生化特性和16S rDNA系统发育分析,确定该菌株为凝结芽孢杆菌。对该菌株胃肠道耐受特性和安全性进行了研究,结果表明,W-02菌株在人工胃液、人工肠液、猪胆盐和80℃高温表现出很强的耐受能力,急性毒性试验结果表明该菌株为无毒菌株。
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| 关 键 词： | 凝结芽孢杆菌 鉴定 猪肠道 生物学特性 |
Isolation and identification of probiotics from swine intestine and its biological characteristics |
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| GAO Shu-feng
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LIU Hui-zhi
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ZHOU Ying-hua
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MIAO Dong
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WU Sheng-lian
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ZHOU Xiao-ling.Isolation and identification of probiotics from swine intestine and its biological characteristics[J].Animal Husbandry & Veterinary Medicine,2012,44(1):19-23. |
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| Authors: | GAO Shu-feng LIU Hui-zhi ZHOU Ying-hua MIAO Dong WU Sheng-lian ZHOU Xiao-ling |
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| Institution: | (Hunan Institute of Microbiology,Changsha 410009,China) |
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| Abstract: | A bacterial strain,named W-02,with strong antagonistic effect on pathogens of swine colibacilosis and salmonellosis was isolated from healthy swine intestine.On the basis of bacterial culture,physiological and biochemical characteristics and 16S rDNA sequences analysis,W-02 strain was identified as Bacillus coagulan.After being inoculated in artificial gastric juice and intestinal liquid for different time,W-02 strain showed stronger tolerance.And the bacterial strain had higher tolerance to different swine bile concentrations and high temperature of 80℃ for different time.Acute toxicity test demonstrated W-02 strain to be avirulent. |
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| Keywords: | Bacillus coagulan identification swine intestine biological characteristics |
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