| 新疆绵羊及其环境中李氏杆菌的生态分布多样性调查 |
| |
| 引用本文： | 赵新斐,尚文婧,王静梅,剡根强.新疆绵羊及其环境中李氏杆菌的生态分布多样性调查[J].畜牧与兽医,2012,44(1):28-32. |
| |
| 作者姓名： | 赵新斐 尚文婧 王静梅 剡根强 |
| |
| 作者单位： | 石河子大学动物科技学院,新疆石河子,832000 |
| |
| 摘 要： | 对新疆健康绵羊及其相关环境如青贮饲料、饮水、土壤、鸟粪中李氏杆菌(Listeria)种群采用改良国标法(4789.30-2010)进行生态分布多样性调查,并对分离的单核细胞增多性李氏杆菌(L.monocytogenes)采用PCR方法进行溶血素基因(hly)和血清型检测。结果:514份样本中,共检出阳性样本26份,平均阳性率为5.1%,其中羊体分离率为6.2%(22/353),饮水分离率为33%(2/6),鸟粪分离率为3.0%(2/67),其余样本均为阴性;26份阳性样品中,检出单核细胞增多性李氏杆菌5株,塞氏李氏杆菌(L.seeligeries)21株,且5株单核细胞增多性李氏杆菌均检出溶血素基因,血清型为1/2a。
|
| 关 键 词： | 李氏杆菌 生态分布 绵羊 环境 |
Ecological distribution of Listeria spp.in healthy sheep and surrounding environments in Xinjiang |
| |
| ZHAO Xin-fei
,
SHANG Wen-jing
,
WANG Jing-mei
,
YAN Gen-qiang.Ecological distribution of Listeria spp.in healthy sheep and surrounding environments in Xinjiang[J].Animal Husbandry & Veterinary Medicine,2012,44(1):28-32. |
| |
| Authors: | ZHAO Xin-fei SHANG Wen-jing WANG Jing-mei YAN Gen-qiang |
| |
| Institution: | (College of Animal Science and Technology,Shihezi University,Shihezi 832000,China) |
| |
| Abstract: | A survey on ecological distribution of Listeria spp.in healthy sheep and surrounding environments such as silage,water,soil and bird feces,was performed using the modified GB method(4789.30-2010) in Xinjiang.For the L.monocytogenes isolates,the hemolysin gene(hly) and serotypes were investigated by PCR method.The result showed that among 514 samples,there were 26 positive specimens,and the average positive rate was 5.1%.The positive rates in sheep,water and bird feces were 6.2%(22/353),33%(2/6) and 3%(2/67),respectively.The remaining samples were negative.Among 26 positive samples,5 were L.monocytogenes and 21 were L.seeligeries.All the five strains of L.monocytogenes were detected hly gene and determined to be the serotype 1/2a. |
| |
| Keywords: | Listeria ecological distribution sheep surrounding environments |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
