| 间接免疫荧光方法快速检测番鸭呼肠孤病毒病 |
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| 引用本文： | 高春亮,蔡羲,朱小丽,陈少莺.间接免疫荧光方法快速检测番鸭呼肠孤病毒病[J].畜牧兽医杂志,2012,31(4):8-9,13. |
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| 作者姓名： | 高春亮 蔡羲 朱小丽 陈少莺 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013
2. 福建省农业科学院畜牧兽医研究所,福州350013 福建省畜禽疫病防治工程技术研究中心 |
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| 基金项目： | 福建省农业科学院创新团队，福建省畜牧重大专项，省公益类项目，福建省星火计划 |
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| 摘 要： | 本研究应用具有免疫荧光特性的抗番鸭呼肠孤病毒病单克隆抗体(MDRV 237-11)建立了检测番鸭呼肠孤病毒病抗原的间接免疫荧光(Mab-IFA)方法。结果显示人工感染MDRVMW9710株病毒后发病和死亡番鸭组织切片均可检出阳性病灶;且组织匀浆经尿囊腔途径接种12日龄番鸭胚,取死亡胚心制作冰冻切片进行IFA鉴定呈阳性结果。表明该方法特异性好、可用于快速检测番鸭呼肠孤病毒病。
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| 关 键 词： | 番鸭呼肠孤病毒病 间接免疫荧光试验 快速检测 |
Rapid Detecting Muscovy Duck Reovirus by Indirect Fluorescence Assay |
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| GAO Chun-liang,CAI Xi,ZHU Xiao-li,CHEN Shao-ying.Rapid Detecting Muscovy Duck Reovirus by Indirect Fluorescence Assay[J].Journal of Animal Science and Veterinary Medicine,2012,31(4):8-9,13. |
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| Authors: | GAO Chun-liang CAI Xi ZHU Xiao-li CHEN Shao-ying |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou Fuj iang 350013, China 2. Fujian Animal Diseases Control Technology Development Center) |
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| Abstract: | Monoclonal antibody（MDRV 237--11）against Muscovy duck reovirus was used to build up indirect fluorescence assay（IFA） in order to detect Muscovy duck reovirus （MDRV）. The results showed that frozen section of Muscovy duck infec- ted with MDRV MW9710 strain could appear specific bright green fluorescence by IFA, moreover, 12--old--day Muscovy duck embryo inoculated with liver and spleen homogenate were isolated the virus. So, IFA could replace virus isolation to detect Muscovy duck reovirus rapidly. |
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| Keywords: | Muscovy duck reovirus indirect fluorescence assay rapid detecting |
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