Characterization of the complex carbohydrates in the zona pellucida of mammalian oocytes using lectin histochemistry |
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| Authors: | F Parillo G Stradaioli C Dall'aglio A Verini-Supplizi |
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| Institution: | (1) Istituto di Anatomia Normale, Facoltà di Medicina Veterinaria, Via San Costanzo 4, 06126 Perugia, Italy;(2) Ostetricia e Ginecologia, Facoltà di Medicina Veterinaria, Via San Costanzo 4, 06126 Perugia, Italy;(3) Produzioni Animali-, Facoltà di Medicina Veterinaria, Via San Costanzo 4, 06126 Perugia, Italy |
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| Abstract: | The objective of this study was to characterize the glycoconjugates present in the zona pellucida of the follicular oocytes in sheep, goats and pigs. The zona pellucida was stained with periodic acid-Schiff, low iron diamine, high iron diamine, and nine different lectin horseradish conjugates: Con-A, SBA, DBA, PNA, RCA-I, GSA-II, WGA, LTA and UEA-I. Staining with DBA, PNA, SBA and RCA-I was performed with and without saponification with KOH and sialidase digestion. The results showed the presence of neutral and acidic glycoconjugates with different terminal sugars and also sialic acid radicals in the zona pellucida of all the animals studied. In particular, the positive staining with WGA, SBA, PNA and RCA-I suggests the presence of oligosaccharides with N-acetyl-d-glucosamine and sialic acid linked to the penultimate  -N-acetyl-d-galactosamine and to the disaccharide galactosyl-(1-3)-N-acetyl-d-galactosamine. The terminal trisaccharide sialic acid galactosyl-(1-4)-N-acetyl-d-glucosamine was identified only in the zona pellucida of ovine and procine oocytes. Thus, the zona pellucida exhibited species-specific variations in the content and distribution of lectin-binding patterns that may reflect the species specificity of gamete interaction.Abbreviations HID
high iron diamine
- HRP
horseradish peroxidase
- LID
low iron diamine
- PAS
periodic acid-Schiff
- PBS
phosphate-buffered saline; see also Tables I and II |
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| Keywords: | carbohydrate glycoconjugates goat lectin oocyte pig sheep zona pellucida |
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