| 姜瘟病菌生防芽孢杆菌蛋白的分离及其生物活性 |
| |
| 引用本文： | 张敏,汤志良,舒凯,陈华保,袁杭,胡晓,赵丹萍.姜瘟病菌生防芽孢杆菌蛋白的分离及其生物活性[J].四川农业大学学报,2010,28(2):196-199. |
| |
| 作者姓名： | 张敏 汤志良 舒凯 陈华保 袁杭 胡晓 赵丹萍 |
| |
| 作者单位： | 四川农业大学农学院,四川,雅安,625014 |
| |
| 基金项目： | 四川省科技厅公益研究计划项目，四川省教育厅项目 |
| |
| 摘 要： | 姜瘟病是由茄科雷尔氏菌(Ralstonia solanacearum)引起的一种细菌性病害,是世界生姜产区中普遍发生的一种毁灭性土传病害。研究表明,从发病田中未发病植株的根际土壤中分离得到的蜡状芽孢杆菌菌株L1(B.cere-us,L1)对茄科雷尔氏菌等8种病原菌具有较强的拮抗能力,具有广谱抗性。经Econo-Pac Q离子交换柱纯化、SDS-PAGE分析,从菌株L1得到一个表观分子量约为40 kDa的拮抗蛋白Lp,该蛋白具有光稳定性,在pH 6.0~8.0,温度20~60℃条件下均表现出对茄科雷尔氏菌的极强拮抗能力,但经胰蛋白酶处理后失活。实验表明,0.38 mg/mL为该蛋白的最低拮抗浓度。
|
| 关 键 词： | 姜瘟病菌 芽孢杆菌 抗菌蛋白 性质 |
Isolation and Characterization of Antagonistic Protein from Bacillus cereus Resistant to Ralstonia solanacearum |
| |
| ZHANG Min,TANG Zhi-liang,SHU Kai,Cheng Hua-bao,YUAN Hang,HU Xiao,ZHAO Dan-ping.Isolation and Characterization of Antagonistic Protein from Bacillus cereus Resistant to Ralstonia solanacearum[J].Journal of Sichuan Agricultural University,2010,28(2):196-199. |
| |
| Authors: | ZHANG Min TANG Zhi-liang SHU Kai Cheng Hua-bao YUAN Hang HU Xiao ZHAO Dan-ping |
| |
| Abstract: | Ginger wilt,caused by Ralstonia solanacearum,is one of the bacterial wilt diseases and a destructive disease in ginger production worldwide.In the present study,L1,an antagonistic bacterium belonging to Bacillus cereus,isolated from the ginger-growing soil,could strongly inhibit the growth of R.solanacearum ect.The antagonistic protein,Lp,produced by L1 was isolated and purified by Econo-Pac Q Ion-exchange chromatography column,and was subjected to the 12% SDS-PAGE and showed only a single band with about 40 kDa.Lp showed the strongest antagonistic activity to R.solanacearum under the conditions of pH 6.0～8.0 and 20～60 ℃.The results also revealed that Lp showed inactivation under light and after being digested by trypsin. |
| |
| Keywords: | Ralstonia solanacearum Bacillus cereus antagonistic protein character |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
