| PEG模拟水分胁迫对桑种萌发及抗性生理生化指标的影响 |
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| 引用本文： | 金筱耘,周金星,余茂德,王茜龄,孙运朋,徐立,李镇刚.PEG模拟水分胁迫对桑种萌发及抗性生理生化指标的影响[J].蚕学通讯,2008,28(4):17-21. |
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| 作者姓名： | 金筱耘 周金星 余茂德 王茜龄 孙运朋 徐立 李镇刚 |
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| 作者单位： | 1. 西南大学生物技术学院,重庆北碚,400716
2. 中国林业科学研究院林业研究所,北京,100091 |
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| 基金项目： | 重庆市十一五重点科技攻关计划项目(编号2006AB1002); 财政部“长江流域低山丘陵综合治理专项”[2006]长江4号 |
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| 摘 要： | 以“桂优62号”桑种为材料，在不同浓度高分子量的聚乙二醇（PEG6000）模拟的水分胁迫条件下，以摇床培养方法对其进行萌发培养。测定不同浓度PEG溶液（30％、25％、20％和双蒸水）中萌发种子的相关抗性生化指标，实验结果经统计分析表明，当PEG浓度为30％时，萌发种子中的脯氨酸、可溶性蛋白、可溶性糖和丙二醛的含量均极显著地高于CK（双蒸水），说明在高浓度PEG溶液处理下能够萌发的种子具有较强的抗水分胁迫的能力，可以作为桑树抗旱育种的选择依据之一。
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| 关 键 词： | 桑树 聚乙二醇 水分胁迫 发芽率 生理生化指标 |
Effects of PEG-Simulated Water Stress on Seed Germination and Physiological and Biochemical Indicators in Mulberry |
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| JIN Xiao-yun,ZHOU Jin-xing,YU Mao-de,WANG Xi-ling,SUN Yun-peng,XU Li,LI Zhen-gang.Effects of PEG-Simulated Water Stress on Seed Germination and Physiological and Biochemical Indicators in Mulberry[J].Newsletter of Sericultural Science,2008,28(4):17-21. |
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| Authors: | JIN Xiao-yun ZHOU Jin-xing YU Mao-de WANG Xi-ling SUN Yun-peng XU Li LI Zhen-gang |
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| Institution: | JIN Xiao-yun ,ZHOU Jin-xing ,YU Mao-de , WANG Xi-ling ,SUN Yun-peng ,XU Li,LI Zhen-gang(1. College of Biotechnology, Southwest University, Beibei, Chongqing 400716, China ; 2. Forestry Research Institute of Chinese Academy of Forestry (CAF), Beijing 100091, China) |
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| Abstract: | Using the seeds of mulberry cv.Guiyou62 as the experiment material,the study investigated effects of PEG-simulated water stress on its seed germination and physiological and biochemical indicators.The seeds were cultured by shake cultivation in solutions containing PEG at 30%,25% and 20%,with deionized water as the control.Then the contents of proline,soluble protein,soluble glycoprotein and malonaldehyde of the material were measured to evaluate its drought resisting ability of the material.The differences... |
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| Keywords: | Mulberry Polyethylene glycol(PEG) Water stress Germination rate Physiological and biochemical indicator |
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