| 表达猪瘟病毒E2蛋白的复制型水疱性口炎假病毒的拯救 |
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| 引用本文： | 陈景艳,李文雯,钱永华.表达猪瘟病毒E2蛋白的复制型水疱性口炎假病毒的拯救[J].动物医学进展,2009,30(9):7-10. |
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| 作者姓名： | 陈景艳 李文雯 钱永华 |
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| 作者单位： | 西北农林科技大学动物医学院,陕西杨陵,712100 |
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| 摘 要： | 为拯救囊膜表面携带有猪瘟病毒E2蛋白的复制型水疱性口炎假病毒,共转染分别表达水疱性口炎病毒核衣壳蛋白、磷蛋白、聚合酶蛋白以及携带有猪瘟病毒E2基因的复制型水疱性口炎病毒载体于BHK-21细胞中.采用Western blot、间接ELISA以及体外微量中和试验检测和鉴定拯救的假病毒.结果显示,E2蛋白成功地在BHK-21细胞中表达,假病毒VSV/CSFV-E2免疫的Balb/c小鼠血清中有高滴度的抗E2抗体,假病毒诱导产生的抗体在体外可以有效的阻断猪瘟病毒囊膜蛋白E1和E2蛋白假型的病毒HIV-luc/CSFV-E1&E2对PK-15细胞的感染.
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| 关 键 词： | 猪瘟病毒 E2蛋白 水疱性口炎病毒 假型病毒 |
Recovery of Vesicular stomatitis virus Recombinants Expressing E2 Protein of CSFV |
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| CHEN Jing-yan,LI Wen-wen,QIAN Yong-hua.Recovery of Vesicular stomatitis virus Recombinants Expressing E2 Protein of CSFV[J].Progress In Veterinary Medicine,2009,30(9):7-10. |
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| Authors: | CHEN Jing-yan LI Wen-wen QIAN Yong-hua |
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| Institution: | CHEN Jing-yan,LI Wen-wen,QIAN Yong-hua(College of Veterinary Medicine,Northwest A & F University,Yangling,Shaanxi,712100,China) |
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| Abstract: | To rescue vesicular stomatitis virus recombinants expressing E2 protein of CSFV,four plasmids expressing nucleoprotein,phosphoprotein,polymerase and E2 protein were co-transfected into BHK-21 cells.Western blot,indirect ELISA,and microneutralization assay were applied to identify the pseudovirus.Results showed that E2 protein was expressed in cells,antibodies against E2 existed in the sera of Balb/c mice immuned with the pseudovirus.Furthermore,the antibodies can neutralize pseudotyped virus HIV-luc/CSFV-E1... |
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| Keywords: | CSFV E2 protein Vesicular stomatitis virus pseudotyped virus |
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