| Identification and Distribution of Bacillus thuringiensis Isolates from Primeval Forests in Yunnan and Hainan Provinces and Northeast Region of China |
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| 作者姓名： | SU Xu-dong SHU Chang-long ZHANG Jie HUANG Da-fang TAN Jian-xin SONG Fu-ping |
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| 作者单位： | [1]State Key Laboratory of Biology for Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R.China [2]Agricultural University of Hebei, Baoding 071001, P.R.China [3]Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划) |
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| 摘 要： |
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| 关 键 词： | 中国 原始森林 苏云金芽孢杆菌 分布 云南 海南 |
| 收稿时间： | 15 March 2007 |
| 修稿时间： | 2007-03-15 |
Identification and Distribution of Bacillus thuringiensis Isolates from Primeval Forests in Yunnan and Hainan Provinces and Northeast Region of China |
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| SU Xu-dong SHU Chang-long ZHANG Jie HUANG Da-fang TAN Jian-xin SONG Fu-ping.Identification and Distribution of Bacillus thuringiensis Isolates from Primeval Forests in Yunnan and Hainan Provinces and Northeast Region of China[J].Agricultural Sciences in China,2007,6(11):1343-1351. |
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| Authors: | SU Xu-dong SHU Chang-long ZHANG Jie HUANG Da-fang TAN Jian-xin SONG Fu-ping |
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| Institution: | 1. College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China;2. College of Pharmacy, Harbin University of Commerce, Harbin 150076, China;3. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan;4. School of Medicine, Nankai University, Tianjin 300071, China;1. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People’s Republic of China;2. State Key Laboratory of Core Technology in Innovative Chinese Medicine, Pharmaceutical Analysis Institute, Tasly Academy, Tianjin 300410, People''s Republic of China;3. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea;4. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan;5. College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University, Kashgar 844000, People’s Republic of China |
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| Abstract: | Ninety-two Bacillus thuringiensis isolates were screened from 683 soil samples collected from tropical and semitropical primeval forests in Yunnan and Hainan provinces of China. Several shapes of crystals, including bipyramidal, square, ovoid, spherical, and amorphous, were observed in the B. thuringiensis isolates. Twenty-six pairs of primers were used to identify 31 holotype cry genes at primary rank of the B. thuringiensis cry gene nomenclature system. The cry gene-types of 92 B. thuringiensis isolates and 33 B. thuringiensis isolates screened from Northeast region of China were identified by PCR-RFLP and SDS-PAGE methods. Fifty-eight isolates harbored cry1 genes, 32 isolates cry2 genes, 12 isolates cry8 genes, 3 isolates cry9 genes, 12 isolates cry11 genes, and 13 isolates cry30 genes. Of the tested isolates, 42 produced no reaction product with 26 pairs of primers and also exhibited no toxicity against 8 insect species tested. The isolate Z2-34 harbored a novel cry30 gene, exhibited insecticidal activity against Aedes albopictus of Dipterans. The accession number of the novel genes in this study is AY916046. Isolation and identification of B. thuringiensis and cry gene are important for investigating the diversity of B. thuringiensis resources and cloning new cry gene. |
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| Keywords: | Bacillus thuringiensis PCR-RFLP cry gene Aedes albopictus |
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