| Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine |
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| 作者姓名： | JING Zhi-zhong DOU Yong-xi LUO Qi-hui CHEN Guo-hua MENG Xue-lian ZHENG Ya-dong LUO Xue-nong CAI Xue-peng |
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| 作者单位： | [1]Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China [2]College of Animal Technology, Sichuan Agricultural University, Yaan 625014, P.R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划);甘肃省重点科技计划项目 |
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| 摘 要： |
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| 关 键 词： | 分子克隆 猪 白介素 同源分析 基因表达 |
Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine |
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| JING Zhi-zhong DOU Yong-xi LUO Qi-hui CHEN Guo-hua MENG Xue-lian ZHENG Ya-dong LUO Xue-nong CAI Xue-peng.Molecular Clone, Expression, and Prediction of Construction and Function to Key Genes of Interleukin Family of Porcine[J].Agricultural Sciences in China,2007,6(7):869-876. |
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| Authors: | JING Zhi-zhong DOU Yong-xi LUO Qi-hui CHEN Guo-hua MENG Xue-lian ZHENG Ya-dong LUO Xue-nong CAI Xue-peng |
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| Institution: | 1. Key Laboratory of Veterinary Parasitology of Gansu Province/State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China
2. College of Animal Technology, Sichuan Agricultural University, Yaan 625014, P.R. China |
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| Abstract: | This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper. |
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| Keywords: | porcine interleukin gene family analysis of homologies prediction of construction |
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