|
|||||
|
|
| Analysis of the Germplasm Resources and Genetic Relationships Among Hybrid Cymbidium Cultivars and Native Species with RAPD Markers | |
| 作者姓名： | LI Dong-mei YE Qing-sheng ZHU Gen-fa |
| 作者单位： | [1]Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China [2]Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China |
| 基金项目： | This research was financially supported by the Science and Technology Key Project of Guangdong Province （2003A201040） and the Agricultural 0ffice of Public Bidding Project of Guangzhou Municipality, China （GK0302105）. |
| 摘 要： | The random amplified polymorphic DNA （RAPD） marker was assessed to detect the genetic relationships among 48 hybrid Cymbidium cultivars from Japan, Korea, China, and USA, and 2 species of native Cymbidium. Twenty primers were screened from 100 random decamer primers, and a total of 258 DNA bands were amplified, 253 of which （98.1%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.6. All cultivars were distinguishable when a number of primers were considered. Genetic similarities among the cultivars and species were estimated based on the amount of band sharing ranging from 0.364-0.817 with an average of 0.581. According to the data, a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and native species were classified into five cluster groups with the similarity coefficient of 0.592. It revealed that the genetic relationships among tested accessions were to some extent related with their origin, flower colour, branch type, and genealogy. It further indicated that the RAPD technique is a useful tool for studying the genetic relationships among hybrid Cymbidium cultivars. |
| 关 键 词： | 兰花 遗传关系 RAPD标记 聚类分析 |
Analysis of the Germplasm Resources and Genetic Relationships Among Hybrid Cymbidium Cultivars and Native Species with RAPD Markers |
|
| LI Dong-mei YE Qing-sheng ZHU Gen-fa.Analysis of the Germplasm Resources and Genetic Relationships Among Hybrid Cymbidium Cultivars and Native Species with RAPD Markers[J].Agricultural Sciences in China,2007,6(8):922-929. | |
| Authors: | LI Dong-mei YE Qing-sheng ZHU Gen-fa |
| Institution: | 1. Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China;Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China 2. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China 3. Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China |
| Abstract: | The random amplified polymorphic DNA (RAPD) marker was assessed to detect the genetic relationships among 48hybrid Cymbidium cultivars from Japan, Korea, China, and USA, and 2 species of native Cymbidium. Twenty primers were screened from 100 random decamer primers, and a total of 258 DNA bands were amplified, 253 of which (98.1%) were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 12.6. All culfivars were distinguishable when a number of primers were considered. Genetic similarities among the cultivars and species were estimated based on the amount of band sharing ranging from 0.364-0.817 with an average of 0.581. According to the data,a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and native species were classified into five cluster groups with the similarity coefficient of 0.592. It revealed that the genetic relationships among tested accessions were to some extent related with their origin, flower colour, branch type, and genealogy. It further indicated that the RAPD technique is a useful tool for studying the genetic relationships among hybrid Cymbidium cultivars. |
| Keywords: | Cymbidium genetic relationship RAPD markers cluster analysis |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ | |