Isolation,growth and differentiation of equine mesenchymal stem cells: effect of donor,source, amount of tissue and supplementation with basic fibroblast growth factor |
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| Authors: | Silvia Colleoni Emanuela Bottani Irene Tessaro Gaetano Mari Barbara Merlo Noemi Romagnoli Alessandro Spadari Cesare Galli Giovanna Lazzari |
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| Institution: | 1.Laboratorio di Tecnologie della Riproduzione,Cremona,Italy;2.Dipartimento Clinico Veterinario,Università di Bologna,Ozzano Emila,Italy |
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| Abstract: | Mesenchymal stem cells (MSC) are increasingly used as therapeutical aid for the orthopaedic injuries in the horse. MSC populate
different tissues but the most commonly used for clinical purposes are isolated from bone marrow or adipose tissue. The first
objective of this study was to investigate if the donor animal, the tissue of origin and the technique of isolation could
influence the number of MSC available for transplantation after a short-term expansion. The second aim was to devise a culture
system capable of increasing MSC lifespan and we tested the effect of basic fibroblast growth factor (bFGF). Results indicate
that MSC can be efficiently isolated from both sources and supplementation of bFGF enhances proliferation rate maintaining
differentiation potential. In addition, this study shows that collection, expansion and storage of frozen MSC can be performed
for later therapeutic use. |
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