| 兰州大尾羊解偶联蛋白3(UCP3)基因荧光定量PCR检测方法的研究 |
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| 引用本文： | 徐红伟,臧荣鑫,杨具田,卢建雄,蔡勇,石来文,马伟龙.兰州大尾羊解偶联蛋白3(UCP3)基因荧光定量PCR检测方法的研究[J].黑龙江畜牧兽医,2010(11). |
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| 作者姓名： | 徐红伟 臧荣鑫 杨具田 卢建雄 蔡勇 石来文 马伟龙 |
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| 作者单位： | 西北民族大学生命科学与工程学院;西北民族大学实验中心; |
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| 基金项目： | 甘肃省农业生物技术研究与应用开发项目(GNSW-2009-13); 西北民族大学研究生科研创新项目(ycx09061) |
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| 摘 要： | 试验通过提取兰州大尾羊不同组织中总RNA,反转录获得总cDNA样品,建立了用SYBR荧光定量PCR法检测兰州大尾羊解偶联蛋白(UCP3)基因mRNA在不同组织中的相对表达量的检测方法,并进行批内、批间重复性检验。结果表明:UCP3和GADPH基因标准曲线回归方程分别为CtUCP3=-3.2343x+41.6198和CtGADPH=-3.2851x+38.0344,相关系数(r2)分别为0.9958和0.9986,扩增效率分别为103%和97%;批内、批间重复性测定的变异系数分别为小于1.4%和10%。
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| 关 键 词： | 兰州大尾羊 解偶联蛋白3 SYBR GreenⅠ法 |
Establishment of the method for detecting Lanzhou fat-tailed sheep uncoupling protein 3 (UCP3) gene mRNA with fluorescence quantitative ploymerase chain reaction |
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| XU Hong-wei,ZANG Rong-xin,YANG Ju-tian,LU Jian-xiong,CAI Yong,SHI Lai-wen,MA Wei-long.Establishment of the method for detecting Lanzhou fat-tailed sheep uncoupling protein 3 (UCP3) gene mRNA with fluorescence quantitative ploymerase chain reaction[J].Heilongjiang Animal Science And veterinary Medicine,2010(11). |
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| Authors: | XU Hong-wei ZANG Rong-xin YANG Ju-tian LU Jian-xiong CAI Yong SHI Lai-wen MA Wei-long |
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| Institution: | XU Hong-wei1,ZANG Rong-xin1,YANG Ju-tian1,LU Jian-xiong1,CAI Yong2,SHI Lai-wen1,MA Wei-long1 (1.College of Life Science and Engineering of Northwest University for Nationalities,Lanzhou 730030,China,2.Science Experimental Center of Northwest University for Nationalities,China) |
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| Abstract: | The experiment was conducted to the method for detecting Lanzhou fat-tailed sheep uncoupling protein 3 (UCP3) gene mRNA expression with real-time PCR developed.By isolated total RNA form the fresh omentum,kidney weeks adipose tissue,muscle and fat tissue,the sample was improved the extraction Trizol one-step method and reverse-transcribed to cDNA using random primers as a primer.The results demonstrated that the standard curve were CtUCP3=-3.234 3x+41.619 8 and CtGADPH =-3.285 1x+38.034 4,had good linear de... |
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