The relationship of soil province to molecular characterization and phylogenetic analysis of Cryptosporidium parvum isolated from calves in Georgia |
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| Authors: | Miller Debra L Mauel Michael J Liggett Alan Hines Murray E Frazier Kendall S Pence Mel Whittington Lisa Baldwin Charles A |
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| Institution: | Veterinary Diagnostic and Investigational Laboratory, College of Veterinary Medicine, The University of Georgia, 43 Brighton Road, Tifton, GA 31793, USA. millerdl@uga.edu |
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| Abstract: | Although Cryptosporidium spp. are found throughout the world and in multiple environmental conditions, few data are available that explore the possibility of an association between specific environmental parameters and the species or strain of Cryptosporidium. This study examines the potential association between a particular Cryptosporidium species/strain found in calves and soil provinces in Georgia, USA. Necropsy cases spanning the years 1996-2002 were tested. No significant differences (P=0.962, chi(2) test of homogeneity) between numbers of positive cases were noted among soil provinces. Phylogenetic analysis of the sequences for the PCR products revealed sequence similarity of the products with Cryptosporidium parvum strain C1. Although, clinical Cryptosporidiosis in calves was not found to be affected by soil province and may be caused by a single genotype, other genotypes may be responsible for subclinical infection and warrant further investigation. |
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| Keywords: | Cryptosporidium parvum Soil PCR Phylogenetic analysis |
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