| 粉纹夜蛾(Trichoplusia ni)卵巢细胞系克隆株Tn5B_(1-4)特性的研究 |
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| 引用本文： | 李国勋.粉纹夜蛾(Trichoplusia ni)卵巢细胞系克隆株Tn5B_(1-4)特性的研究[J].东北农业大学学报,1993(4). |
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| 作者姓名： | 李国勋 |
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| 作者单位： | 东北农学院植物保护系 |
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| 摘 要： | 通过有限稀释法由粉纹夜蛾(Trichoplusia ni)Tn 5 B_1细胞系中分离出多个细胞株,经筛选获得一个理想的克隆株Tn 5 B_(1-4),该克隆株的特性不同于原细胞系Tn_5B_1。该克隆株的细胞形态一致,细胞群体倍增时间较原细胞株短.而且细胞活力强,特别是在维持粉纹夜蛾单粒包埋核型多角体病毒(Tn SNPV)的复制能力方面高于原细胞系。另外经生物测定看出,在该克隆株中增殖的病毒毒力与虫体内增殖的病毒无显著差异。
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| 关 键 词： | 粉纹夜蛾 单粒包埋核型多角体病毒 昆虫细胞系 细胞克隆 |
CHARACTERIZATION OF CLONAL STRAIN Tn5B_(1-4) FROM TRICHOPLUSIA NI OVARY CELL LINE Tn5B_1 |
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| Li Guoxun.CHARACTERIZATION OF CLONAL STRAIN Tn5B_(1-4) FROM TRICHOPLUSIA NI OVARY CELL LINE Tn5B_1[J].Journal of Northeast Agricultural University,1993(4). |
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| Authors: | Li Guoxun |
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| Abstract: | A clonal strain Tn5B_(1-4)was successfully isolated from the established Trichoplusia ni cell line Tn5B, by the method of finite dilution. The growth characteristics of the clonal strain were different from the parent cell line in its morphology, kinetics of cells, cell doubling time and the relative ability to support replication of TnSNPV. The bioassay of TnSNPV from Trichoplusia ni larvae and cloned strain was conducted for comparing their virulence. |
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| Keywords: | Trichoplusia ni SNPV insect cell line cell clone |
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