| 紫外分光光度法测定甘草渣中总黄酮含量 |
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| 引用本文： | 张腊腊,胡浩斌,王宗博,武芸,韩明虎.紫外分光光度法测定甘草渣中总黄酮含量[J].安徽农业科学,2017,45(9). |
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| 作者姓名： | 张腊腊 胡浩斌 王宗博 武芸 韩明虎 |
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| 作者单位： | 陇东学院化学化工学院,甘肃庆阳,745000;庆阳市中凯农产品有限责任公司,甘肃庆阳,745000 |
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| 基金项目： | 甘肃省科技支撑项目，甘肃省应用化学省级重点学科建设项目，陇东学院青年基金项目 |
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| 摘 要： | 目的]建立甘草渣中总黄酮含量的测定方法。方法]以芦丁作为对照品,10%KOH为显色剂,采用紫外分光光度法,在409 nm波长处对样品中的总黄酮含量进行测定。结果]总黄酮在一定范围内呈良好的线性关系,R~2为0.994 5;平均加样回收率为88%,RSD为1.3%,甘草渣中总黄酮的含量为2.24%。结论]该方法稳定、简便,适用于甘草渣中总黄酮含量的测定。
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| 关 键 词： | 紫外分光光度法 甘草渣 总黄酮含量 |
Determination of Total Flavonoids in Licorice Residue by UV Spectrophotometry |
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| Abstract: | Objective] The research aimed to establish the determination method of total flavonoids content in licorice residue.Method]Using rutin as the reference substance and 10% KOH as the color reagent,the content of total flavonoids in the samples was measured at 409 nm by UV spectrophotometry.Result]The total flavonoids showed a good linearity in a certain range,R2 was 0.994 5.The average recoveries were 88%,RSD was 1.3%,and the content of total flavonoids in licorice residue was 2.24%.Conclusion] The method is stable and simple,and is suitable for the determination of total flavonoids in licorice residue. |
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| Keywords: | UV spectrophotometry Licorice residue Total flavonoids content |
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