| 不同抗凝剂对鲫鱼外周血的抗凝效果研究 |
| |
| 引用本文： | 刘凯,谢楠,马恒甲,姚桂桂.不同抗凝剂对鲫鱼外周血的抗凝效果研究[J].安徽农业科学,2017,45(8). |
| |
| 作者姓名： | 刘凯 谢楠 马恒甲 姚桂桂 |
| |
| 作者单位： | 杭州市农业科学研究院,浙江杭州,310024;杭州市农业科学研究院,浙江杭州,310024;杭州市农业科学研究院,浙江杭州,310024;杭州市农业科学研究院,浙江杭州,310024 |
| |
| 基金项目： | 国家大宗淡水鱼产业技术体系项目,杭州市科委种子种苗项目 |
| |
| 摘 要： | 目的]研究不同抗凝剂对鲫鱼外周血的抗凝效果。方法]以鲫鱼"中科3号"为研究对象,比较5种抗凝剂对鲫鱼外周血的抗凝效果,探讨其适宜抗凝剂品种和有效抗凝浓度。结果]除了L-半胱氨酸在试验条件下不能作为有效抗凝剂外,其余4种抗凝剂延长血液凝固时间均在61.43 min以上。肝素钠、柠檬酸钠、草酸钾和EDTA的有效抗凝浓度分别为(0.80±0.17)、(1.42±0.14)、(1.17±0.14)和(2.33±0.58)mg/mL。显微镜下,肝素钠、柠檬酸钠、草酸钾和EDTA 4种抗凝剂均能较好地维持鲫鱼外周血细胞的形态。结论]该研究结果可为鲫鱼外周血抗凝剂的应用提供参考。
|
| 关 键 词： | 鲫鱼 外周血 抗凝剂 抗凝效果 |
Study on the Anticoagulation Effect of Different Anticoagulants on Peripheral Blood of Crucian Carp |
| |
| Abstract: | Objective] To study the anticoagulation effect of different anticoagulants on peripheral blood of crucian carp.Method] Using crucian carp cultivar "Zhongke No.3" as research object,the anticoagulation effects of five kinds of anticoagulants on peripheral blood of crucian carp were compared.The suitable kind of anticoagulant and its effective concentration for peripheral blood of crucian carp were discussed.Result] Under these test conditions,except for L-cysteine,other four kinds of anticoagulants could prolong the blood coagulation time more than 61.43 min.The effective anticoagulation concentrations of heparin sodium,sodium citrate,potassium oxalate and EDTA were (0.80±0.17),(1.42±0.14),(1.17±0.14) and (2.33±0.58) mg/mL respectively.Under the microscope,the hemocyte morphology of peripheral blood in crucian carp treated with heparin sodium,sodium citrate,potassium oxalate and EDTA could be well maintained.Conclusion] The research can provide references for the application of anticoagulants for peripheral blood of crucian carp. |
| |
| Keywords: | Crucian carp Peripheral blood Anticoagulant Anticoagulation effect |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
