| 动物双歧杆菌AD011表面脂蛋白的全基因组预测和功能分析 |
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| 引用本文： | 栗金柳,王茗悦,朱德全,高峰,于陆雪子,周金影,郝莹莹,张宛麟,周博.动物双歧杆菌AD011表面脂蛋白的全基因组预测和功能分析[J].安徽农业科学,2017,45(11). |
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| 作者姓名： | 栗金柳 王茗悦 朱德全 高峰 于陆雪子 周金影 郝莹莹 张宛麟 周博 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学农业与环境生物技术研究所,黑龙江佳木斯,154007;佳木斯大学信息电子技术学院,黑龙江佳木斯,154007 |
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| 基金项目： | 黑龙江大学生创新创业项目，佳木斯大学大学生科技创新项目，黑龙江省卫生计生委科研项目 |
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| 摘 要： | 目的]运用生物信息学方法预测动物双歧杆菌AD011定位于细胞膜的表面脂蛋白,为后续研究该菌与宿主相互作用的分子机制提供基础资料。方法]用Signal P 4.0和Lipo P软件对动物双歧杆菌AD011全基因组编码蛋白中表面脂蛋白和信号肽进行预测,同时采用COG功能数据库对预测的脂蛋白进行功能注释和聚类分析。结果]对动物双歧杆菌AD011基因组1 518个蛋白的表面脂蛋白预测,结果表明,19个蛋白具有脂蛋白信号肽。功能分析显示,该菌株19个脂蛋白中有3个蛋白没有功能注释,16个蛋白有功能注释,其中10个蛋白参与氨基酸代谢与转运、3个蛋白参与碳水化合物代谢与转运,3个蛋白参与无机盐离子代谢与转运。结论]动物双歧杆菌AD011表面脂蛋白参与的功能主要与营养物质的转运和代谢有关。
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| 关 键 词： | 动物双歧杆菌 基因组 脂蛋白 信号肽 |
Genome-wide Prediction and Functional Analysis of Surface Lipoproteins of Bifidobacterium animalis AD011 |
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| Abstract: | Objective] The surface lipoproteins of Bifidobacterium animalis AD011 were predicted by bioinformatics method,which provided the basis for interaction molecular mechanisms between bacteria and their host.Method] SignalP 4.0 and LipoP softwares were used to analyze the lipoproteins and characteristic of signal peptides.Function of lipoproteins was also analyzed by COG database.Result] There were 19 proteins which possessed signal peptide of lipoproteins from 1 518 proteins coded by B.animalis AD011 genome.Function analysis revealed that the strain contains 3 hypothetical proteins without function annonation.16 proteins have function annonation which mainly involved in amino acid metabolism and transport(10 proteins),transport of carbohydrate metabolism(3 proteins),metabolism of inorganic ion transport(3 proteins).Conclusion] Function of surface lipoproteins of B.animalis AD011 are mainly associated with the transport and metabolism of nutrients. |
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| Keywords: | Bifidobacterium animalis Genome Lipoproteins Signal peptide |
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