| 动物双歧杆菌AD011暴露外表面蛋白的种类和功能分析 |
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| 引用本文： | 朱德全,王茗悦,栗金柳,张跃华,张武.动物双歧杆菌AD011暴露外表面蛋白的种类和功能分析[J].安徽农业科学,2017,45(11). |
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| 作者姓名： | 朱德全 王茗悦 栗金柳 张跃华 张武 |
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| 作者单位： | 佳木斯大学农业与环境生物技术研究所,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007 |
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| 基金项目： | 黑龙江省卫生计生委科研项目 |
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| 摘 要： | 目的]分析动物双歧杆菌AD011暴露外表面蛋白的种类和功能,有助于了解该菌株的各项生理功能以及为探讨该菌与宿主相互作用的分子机制奠定基础。方法]以动物双歧杆菌AD011基因组序列为研究对象,采用Inmembrane方法和COG功能数据库对预测的外表面蛋白进行功能注释和聚类分析。结果]AD011菌体暴露外表面蛋白数目为193个,包括11个细胞壁蛋白、20个脂蛋白、暴露外表面的膜蛋白140个、22个细胞外蛋白。AD011外表面蛋白的功能分析结果显示,193个外表面蛋白中,含有较大比例的蛋白没有功能注释(78个),115个蛋白具有COG功能注释。在有功能注释的蛋白中,所占比例较大的是氨基酸转运与代谢(16个),无机离子转运与代谢(14个),细胞壁、细胞膜生物合成(13个),碳水化合物转运与代谢(10个),防御机制(10个)等有关蛋白。结论]AD011菌体外表面蛋白与营养物质的吸收和转运,细胞壁、细胞膜生物合成,防御机制有关。
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| 关 键 词： | 动物双歧杆菌 基因组 外表面蛋白 种类 功能 |
Species and Functional Analysis of the Outer Surface Proteins of Bifidobacterium animalis AD011 |
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| ZHU De-quan,WANG Ming-yue,LI Jin-liu,ZHANG Yue-hua,ZHANG Wu.Species and Functional Analysis of the Outer Surface Proteins of Bifidobacterium animalis AD011[J].Journal of Anhui Agricultural Sciences,2017,45(11). |
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| Authors: | ZHU De-quan WANG Ming-yue LI Jin-liu ZHANG Yue-hua ZHANG Wu |
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| Abstract: | Objective]Species and function of the outer surface proteins of Bifidobacterium animalis AD011 were analyzed.Analysis of their outer surface proteins will help us to correctly understand its physiological function and molecular mechanisms of interaction with their host.Method]The genome sequence of B.animalis AD011 was used to study the potentially surface exposed proteins (PSE proteins) by Inmembrane.Functional annotation and cluster analysis of predicted surface proteins were performed using COG functional database.Result]There were 193 surface exposed proteins which include 11 cell wall proteins,20 lipoproteins,140 membrane proteins and 22 extracellular proteins.Results of functional analysis showed there were 78 proteins which have not COG functional annotation in 193 surface exposed proteins.Most outer surface proteins were involved in amino acid transport and metabolism(16 proteins),inorganic ion transport and metabolism(14 proteins),cell wall/membrane formation(13 proteins),carbohydrate transport and metabolisms(10 proteins),defense mechanisms(10 proteins).Conclusion]The function of outer surface proteins of AD011 were involved in absorption and transport of nutrient,cell wall/membrane biosynthesis and defense mechanisms. |
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| Keywords: | Bifidobacterium animalis Genome Outer surface protein Species Function |
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