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| 监测重组蛋白EgM9免疫犬的特异性抗体变化 | |
| 引用本文： | 赵莉,张壮志,石保新,古努尔·吐尔逊,米晓云,吐尔洪·依米提,王进成,马正海,张文宝.监测重组蛋白EgM9免疫犬的特异性抗体变化[J].新疆农业科学,2011,48(6):1115-1119. |
| 作者姓名： | 赵莉 张壮志 石保新 古努尔·吐尔逊 米晓云 吐尔洪·依米提 王进成 马正海 张文宝 |
| 作者单位： | 1. 新疆大学生命科学与技术学院,乌鲁木齐,830000;新疆畜牧科学院兽医研究所,乌鲁木齐,830000 2. 新疆畜牧科学院兽医研究所,乌鲁木齐,830000 3. 新疆大学生命科学与技术学院,乌鲁木齐,830000 |
| 基金项目： | 国家自然科学基金项目，美国NIH基金资助项目 |
| 摘 要： | 【目的】探索EgM家族重组蛋白(EgM9)与Iscom为佐剂结合免疫犬后血清抗体变化规律。【方法】利用间接ELISA法检测免疫组与对照组血清特异性抗体(IgGI、gG1I、gG2、IgA、IgM和IgE)的变化规律,并以Western-blotting方法进行验证。【结果】经统计学方法分析显示,免疫组与对照组特异性抗体IgG及其亚类IgG1I、gG2,差异极显著(P<0.01),而特异性抗体IgAI、gM和IgE差异不显著(P>0.05)。【结论】证实EgM家族重组蛋白(EgM9)与Iscom为佐剂结合能引起犬特异性免疫应答。 |
| 关 键 词： | 重组蛋白EgM9 特异性抗体 犬 |
Detection of the Specific Antibodies Variation in Vaccinated Dogs by Recombinant Protein EgM9 with Adjuvant Iscom |
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| ZHAO Li,ZHANG Zhuang-zhi,SHI Bao-xin,Gulnur Tursun,MI Xiao-yun,Turbon Yimit,WANG Jin-cheng,MA Zheng-hai,ZHANG Wen-bao.Detection of the Specific Antibodies Variation in Vaccinated Dogs by Recombinant Protein EgM9 with Adjuvant Iscom[J].Xinjiang Agricultural Sciences,2011,48(6):1115-1119. | |
| Authors: | ZHAO Li ZHANG Zhuang-zhi SHI Bao-xin Gulnur Tursun MI Xiao-yun Turbon Yimit WANG Jin-cheng MA Zheng-hai ZHANG Wen-bao |
| Institution: | ZHAO Li1,2,ZHANG Zhuang-zhi2,SHI Bao-xin2,Gulnur Tursun2,MI Xiao-yun2,Turbon Yimit2,WANG Jin-cheng2,MA Zheng-hai1,ZHANG Wen-bao 2(1.College of Life Science and Technology,Xinjinag University,Urumqi 830000,China,2.Veterinary Research Institute of Animal Science Academy,Xinjiang Uygur Autonomous Region,China) |
| Abstract: | 【Objective】The purpose of this study is to test the antibodies of the dogs induced by the recombinant EgM family protein(EgM9) with the Iscom as an adjuvant.【Method】 The levels of special antibodies(IgG,IgG1,IgG2,IgA,IgM and IgE) were detected by indirect ELISA.【Result】 The EgM group was extremely different from the control group in the specific antibodies,IgG1 and IgG2(P<0.01),but there was no significant difference in the specific antibody IgA,IgM and IgE(P>0.05).【Conclusion】 Recombinant EgM family protein(EgM 9) caused specific immune response in dogs with Iscom as an adjuvant. |
| Keywords: | the EgM family recombinant protein(EgM9) specific antibody dog |
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