| 新疆库米什金矿土壤可培养细菌的多样性分析 |
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| 引用本文： | 艾尼江·尔斯曼,玛丽帕·吐达洪,马丽艳木·阿木东,卡依尔·玉素甫,祖母拉提·阿布都热依木,程刚,艾尔肯·热合曼.新疆库米什金矿土壤可培养细菌的多样性分析[J].新疆农业科学,2011,48(6):1167-1173. |
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| 作者姓名： | 艾尼江·尔斯曼 玛丽帕·吐达洪 马丽艳木·阿木东 卡依尔·玉素甫 祖母拉提·阿布都热依木 程刚 艾尔肯·热合曼 |
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| 作者单位： | 新疆大学生命科学与技术学院,乌鲁木齐,830046 |
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| 基金项目： | 新疆维吾尔自治区科技支疆项目，国家自然科学基金，新疆特殊环境微生物实验室开放课题 |
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| 摘 要： | 目的]了解新疆库米什一处金矿土壤可培养细菌的多样性及建立菌种资源库.方法]采集库米什金矿土壤样品,采用两种不同培养基(LB,TSB)分离纯化细菌,对其进行形态观察(菌落及细胞)、生理测定、16SrDNA测序和系统进化分析.结果]从两种培养基上总共得到50株细菌,根据基于16S rDNA的系统进化分析表明,50株菌...
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| 关 键 词： | 库米什 金矿 可培养细菌 16S rDNA序列 多样性 |
Analysis of the Diversity of Culturable Bacteria in the Soil from Kumishi Gold Mine,Xinjiang |
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| Ainijan Osman,Maripat Turdahon,Maryam Armdun,Khayir Yusuf,Zumret Abdurehim,CHENG Gang,Erkin Rahman.Analysis of the Diversity of Culturable Bacteria in the Soil from Kumishi Gold Mine,Xinjiang[J].Xinjiang Agricultural Sciences,2011,48(6):1167-1173. |
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| Authors: | Ainijan Osman Maripat Turdahon Maryam Armdun Khayir Yusuf Zumret Abdurehim CHENG Gang Erkin Rahman |
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| Institution: | Ainijan Osman,Maripat Turdahon,Maryam Armdun,Khayir Yusuf,Zumret Abdurehim,CHENG Gang,Erkin Rahman(College of Life Science and Technology,Xinjiang University,Urumqi,830046,China) |
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| Abstract: | 【Objective】The purpose of this study is to understand the diversity of cultivable bacteria in the soil from a gold mine in Kumishi Town,Xinjiang and build a strain repository.【Method】 Bacteria were isolated from the soil of the gold mine by using 2 types of different cultural medium(LB,TSB).Their taxonomic positions were determined by using 16S rDNA-based phylogenetic analysis.【Result】 After isolation and purification,a total of 50 bacterial isolates were obtained from the two culture media.According to their 16S rDNA sequence similarities to type strains of validly described organisms,they have been placed into three phylogenetic groups(Firmicutes,Actinobacteria and γ-Proteobacteria),12 genera and 30 species,in which,the genera bacillus is the most predominant and has occupied the majority of isolates(63.3%).Among them,the isolate L10-5 showed the lowest 16S rDNA sequence similarity(96.463%) to its closely related type strain,which was presumed to be a potential novel species.【Conclusion】 The result showed that the cultivable bacteria in the gold mine soil in Kumishi are of high diversity and there might exist potential novel species that need to be further investigated. |
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| Keywords: | Kumishi Town gold mine cultivable bacteria 16S rDNA diversity |
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