| 3种木腐菌木质纤维素相关酶基因的TRAP标记体系优化与遗传变异初探 |
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| 引用本文： | 王玉英,吕世翔,王秋玉.3种木腐菌木质纤维素相关酶基因的TRAP标记体系优化与遗传变异初探[J].林业科技,2012,37(2):17-20. |
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| 作者姓名： | 王玉英 吕世翔 王秋玉 |
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| 作者单位： | 东北林业大学生命科学学院,黑龙江哈尔滨150040〉 |
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| 摘 要： | 以黑龙江省东北林业大学帽儿山实验林场的3种典型木材腐朽菌为试验材料,采用TRAP分子标记的手段,针对5种木质素与纤维素相关酶基因LiP、MnP、Lac、CBHⅡ、CDH设计引物,初步分析这些编码基因的多态性。试验确定了3种木腐菌TRAP分子标记的反应体系和反应程序。经过对32对引物进行筛选试验,共确定11对引物,包括3对标记MnP编码基因的引物、3对标记Lac编码基因的引物、3对标记CBH编码基因的引物,以及2对标记CDH编码基因的引物。结果表明:3种木腐菌的TRAP-PCR标记共产生了265条条带,其中多态性条带206条,占总条带的77.74%,多态性最高为100%,最低为60%,证明了TRAP分子标记可以应用于木腐菌的遗传分析。
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| 关 键 词： | 木材腐朽菌 基因 引物 TRAP 多态性 遗传差异 |
Optimization of TRAP-PCR System and A Primary Study on Genetic Variation of Genes Related to Lignocellulose Enzyme of Three Wood-rotting Fungi |
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| Institution: | WANG Yuying (Northeast Forestry University, Heilongjiang Harbin 150040) |
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| Abstract: | Collecting three typical wood-rotting fungi from Maoer Mountain Forest Farm of Northeast Forestry University as the experimental materials, a preliminary study on polymorphism of the encoding genes of five lignocellulose enzyme, including lip, mnp, lac, cbh II and cdh, have been done. At the same time, the reaction system and process of TRAP-PCR of three wood-rotting fungi have been optimized firstly, and then 11 pair primers were selected from 32 pair primers, including 3 pair primers of MnP encoding gene, 3 pair of Lae encoding gene, 3 pair of CBH encoding gene, as well as 2 pair of CDH encoding gene. The result shows that three wood-rotting fungi generated 265 bands by TRAP-PCR, among which 206 bands are polymorphic bands with 77.74% in potymorphic rate ranging from 60% in minimum and 100% in maximum. This experiment proved the possibility of genetic analysis of wood- rotting fungus by using TRAP marker. |
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| Keywords: | Wood-rotting fungus Gene Primer TRAP Polymorphism Genetic difference |
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