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| 球孢白僵菌侵染光肩星天牛幼虫的扫描电镜及组织病理观察 | |
| 引用本文： | 邓彩萍,闫喜中,刘红霞,郝赤,骆有庆.球孢白僵菌侵染光肩星天牛幼虫的扫描电镜及组织病理观察[J].林业科学,2012,48(3):105-109. |
| 作者姓名： | 邓彩萍 闫喜中 刘红霞 郝赤 骆有庆 |
| 作者单位： | 1. 北京林业大学省部共建森林培育与保护教育部重点实验室 北京100083;山西农业大学林学院 太谷030801 2. 山西农业大学农学院 太谷030801 3. 北京林业大学省部共建森林培育与保护教育部重点实验室 北京100083 |
| 基金项目： | 国家重点基础研究发展计划资助项目，林业公益性行业科研专项，中央高校基本科研业务费专项，山西农业大学引进人才科研启动基金 |
| 摘 要： | 应用扫描电镜和组织切片法研究球孢白僵菌侵染光肩星天牛幼虫体壁的过程以及寄主中肠发生的病理变化.结果表明:接菌后48h,芽管平均长度达到3.08μm时即可侵入幼虫体表.随着侵染时间延长,芽管伸长形成菌丝,逐渐萌发生成新的分生孢子,在后期菌丝间出现丝状物和网状结构,这2种现象在国内外未见报道.分生孢子多数以芽管方式侵入表皮,也可以产生附着胞再侵染.组织切片显示:侵染3天的幼虫腹部体腔中脂肪体组织变得模糊,细胞核不明显,马氏管出现空泡并变形.中肠围食膜被分解消失,肠壁肌肉层向内皱褶,排列紊乱,出现破裂,肠腔内变浑浊. |
| 关 键 词： | 球孢白僵菌 光肩星天牛 侵染过程 病理变化 扫描电镜 |
Pathological Observation of Anoplophora glabripennis Larva Infected by Beauveria bassiana by Using SEM and Light Microscope |
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| Deng Caiping , Yan Xizhong , Liu Hongxia , Hao Chi , Luo Youqing.Pathological Observation of Anoplophora glabripennis Larva Infected by Beauveria bassiana by Using SEM and Light Microscope[J].Scientia Silvae Sinicae,2012,48(3):105-109. | |
| Authors: | Deng Caiping Yan Xizhong Liu Hongxia Hao Chi Luo Youqing |
| Institution: | 1(1.Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University Beijing 100083;2.Forestry College,Shanxi Agricultural University Taigu 030801;3.Agricultural College,Shanxi Agricultural University Taigu 030801) |
| Abstract: | The infection course of Beauveria bassiana in the integument of Anoplophora glabripennis larva was examined by the scanning electronic microscope(SEM),and a histopathological study with paraffin section of the midgut of the host was also conducted by using a light microscope.The result revealed that the germ tube with its length more than 3.08 μm was able to penetrate the integument in 48 h post inoculation.Then the germ tube gradually developed into hypha and new conidium.Some silk-like and net structures were found in the late phase,which had not been reported before.Most of the conidia invaded the cuticle of the host larvae directly with the germ tube,and sometimes an appressorium was produced during the course of infection and invaded the cuticle later.Microscope examination with paraffin section showed that the fat body in the body cavity of the infected larva became unclear and the nucleus was atrophic in 3 days post inoculation.Vacuoles appeared in the Malpighian tubules which caosed the tubules deformed.The peritrophic membrane in the midgut was decomposed and disappeared.The circular muscle layer in the midgut folded inward and was disintegrated.The midgut cavity of the A.glabripennis became turbid. |
| Keywords: | Beauveria bassiana Anoplophora glabripennis infection process histopathology SEM |
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