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| 13株新城疫病毒强毒株的分离鉴定及部分生物学特性 | |
| 引用本文： | 连宏军,闫丽辉,刘培欣,曹殿军,韩正博,孙建宏,梁宏志.13株新城疫病毒强毒株的分离鉴定及部分生物学特性[J].动物医学进展,2006,27(6):64-69. |
| 作者姓名： | 连宏军 闫丽辉 刘培欣 曹殿军 韩正博 孙建宏 梁宏志 |
| 作者单位： | 1. 新疆农垦科学院新疆生产建设兵团绵羊繁育生物技术重点实验室,新疆石河子832000 2. 中国农科院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 3. 哈尔滨市兽医卫生防疫站,黑龙江哈尔滨150004 |
| 摘 要： | 2004年-2005年从黑龙江省11个地区鸡场、鸽场、鹅场疑似新城疫(ND)病死鸡、鸽、鹅体内分离到24株具有血凝活性的病毒,经HA、HI试验和电镜观察证实了13株病毒为NDV。其鸡胚平均死亡时间(MDT)和1日龄雏鸡脑内接种致病指数(IC-PI)分别为36.8 h~73.6 h和1.51~2.00之间,属于NDV强毒株或中强毒株。除HLJ-12-04株外,强毒株的血凝素热稳定性较差,属快速或中速血凝解脱型;弱毒株的血凝素热稳定性好,属慢速血凝解脱型。所有毒株均可较好地凝集鸡和人(O型血)的红细胞,但对其他动物的红细胞凝集性差异较大,对马和兔的红细胞凝集无规律。 |
| 关 键 词： | 新城疫病毒 分离鉴定 生物学特性 |
| 文章编号： | 1007-5038（2006）06-0064-06 |
| 收稿时间： | 2006-02-28 |
| 修稿时间： | 2006年2月28日 |
Isolation and Identification of Velogenic Newcastle disease virus of 13 Strains and Their Biological Characteristics |
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| LIAN Hong-jun,YAN Li-hui,LIU Pei-xin,CAO Dian-jun,HAN Zheng-bo,SUN Jian-hong,LIANG Hong-zhi.Isolation and Identification of Velogenic Newcastle disease virus of 13 Strains and Their Biological Characteristics[J].Progress In Veterinary Medicine,2006,27(6):64-69. | |
| Authors: | LIAN Hong-jun YAN Li-hui LIU Pei-xin CAO Dian-jun HAN Zheng-bo SUN Jian-hong LIANG Hong-zhi |
| Institution: | 1. The Breed and Biotechnology Key Laboratory of Sheep ,Xinjiang Production and Construction Group, Xinjiang Academy of Agricultural Reclamation, Shihexi, Xinjiang, 832000 ,China ; 2. National Key laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute ,CAAS, Harbin, Heilongjiang , 150001, China ; 3. Harbin Veterinary Station, Harbin, Heilongjiang , 150004, China |
| Abstract: | 13 strains of Newcastle disease virus(NDV),11 from vaccinated chickens and 2 from pigeons,were isolated from Heilongjiang Province during 2004-2005.All isolates' mean death time(MDT) of embryonated eggs,interacerebral pathogenicity index(ICPI),hemagglutination spectrum of erythrocytes, and hemagglutination-separation reactions were tested.The results showed that MDT of all NDV isolates were between 36.8 hours and 73.6 hours,ICPI were between 1.51 and 2.00,belonged to velogenic NDV.All 13 strains can agglutinate the erythrocytes of chicken and human O type.However,their ability of agglutinating erythrocytes of swine,horse,cow,rabbit and guinea pig were variable.Accept from HLJ-12-04,the hemagglutinin of lentogenic strains were stable to heat and belonged to slow type of agglutination-separation,virulent strains were not stable to heat and belonged to fast type of agglutination-separation. |
| Keywords: | Newcastle disease virus isolation and identification biological characteristics |
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