| 响应面法优化提取糜子油 |
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| 引用本文： | 田 翔,乔治军,田琴.响应面法优化提取糜子油[J].中国农学通报,2016,32(28):183-188. |
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| 作者姓名： | 田 翔 乔治军 田琴 |
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| 作者单位： | 山西省农业科学院农作物品种资源研究所,农业部黄土高原作物基因与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室 |
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| 基金项目： | 山西省农科院攻关项目“HPLC法测定糜子中的多种水溶性维生素”(ygg1514)；现代农业产业技术体系建设专项资金资助“谷子糜子抗旱耐瘠薄栽培生理研究”(CARS-07-13.5)。 |
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| 摘 要： | 为了确定糜子油的最优提取工艺，并对其脂肪酸成分进行分析，在单因素实验基础上，以糜子油得率作为响应目标，通过中心复合设计进行优化，确定索氏提取法提取糜子油的最佳条件。利用气质联用仪对糜子脂肪酸成分检测。结果表明，提取溶剂石油醚，液料比为20:1，提取温度80℃，淋洗时间30 min 时提取条件为最佳，最佳得率为4.51%。对糜子油进行甲酯化后经气相色谱质谱仪分析，得到其主要脂肪酸为油酸、亚油酸、棕榈酸、亚麻酸等，主要以不饱和脂肪酸为主，是优质脂肪酸。
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| 关 键 词： | 土壤水分 土壤水分 CERES-MAIZE 根系生物量 叶面积指数 |
| 收稿时间： | 3/4/2016 12:00:00 AM |
| 修稿时间： | 2016/9/21 0:00:00 |
Response Surface Methodology Optimizing Extraction Process of Oil from Panicum miliaceum L. |
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| Institution: | (Key Laboratory of Crop Gene Resources & Germplasm Enhancement on Loess Plateau, Ministry of Agriculture/ Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Institute of Crop Germplasm Resources |
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| Abstract: | In order to determine the optimum extraction process of oil fromPanicum miliaceum L., and analyze the fatty acid composition, based on the single factor experiment, oil yield was taken as the response target, central composite design of response surface methodology was used to optimize the extraction conditions, Soxhlet extraction method was used to extract oil. Gas chromatograph-mass spectrometer (GC-MS) was used to detect fatty acid composition of oil fromPanicum miliaceum L.. The results showed that taking petroleum ether as extraction solvent and making liquid-to-solid ratio at 20:1, extracting oil at the temperature of 80℃ and leaching for 30 min could get the best yield, which was 4.51%. GC-MS analysis showed that the main fatty acids of oil fromPanicum miliaceum L. were oleic acid, linoleic acid, palmitic acid and linolenic acid, which were unsaturated and high-quality fatty acids. |
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| Keywords: | oil fromPanicum miliaceum L response surface methodology Soxhletextraction |
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