| 禽流感病毒A/Ostrich/Denmark/72420/96(LPAI-H5N2)株HA全基因克隆与序列分析 |
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| 引用本文： | 李明义,于树涛,刘新文,宫晓,郭玉广,隋国宁.禽流感病毒A/Ostrich/Denmark/72420/96(LPAI-H5N2)株HA全基因克隆与序列分析[J].中国动物检疫,2006,23(10):19-22. |
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| 作者姓名： | 李明义 于树涛 刘新文 宫晓 郭玉广 隋国宁 |
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| 作者单位： | 1. 中国动物卫生与流行病学中心,青岛易邦生物工程有限公司,青岛市,266032
2. 莱阳市畜牧局,265200 |
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| 基金项目： | 科技部科技攻关专项支持项目 |
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| 摘 要： | 为进一步分析禽流感病毒(AIV)H5N2分离株血凝素(HA)基因的特性,参照已发表H5亚型禽流感HA基因序列设计了1对引物,采用RT-PCR技术,以禽流感病毒A/Ostrich/Denmark/72420/96(D96)RNA为模板,扩增了HA全基因并进行核苷酸同源性比较,氨基酸编码分析,绘制系统发育进化树。结果表明,扩增片段长1737个核苷酸,包含了完整的HA基因的开放阅读框架,与Genbank已发表的H5N1和H5N2分离株的HA基因序列比较,发现与国内H5N1分离株同源性较低,只有80%左右,而与H5N2各株序列具有很高的同源性,最高达97.5%,印证了AIV基因组8个片段间频繁的重组及AIV高变异性的特点。推导的氨基酸序列分析表明,HA蛋白裂解位点上游丢失了4个连续碱性氨基酸(R-R-R-K),裂解位点处氨基酸序列为E-T-R,仅包含一个碱性氨基酸(R-)残基,符合低致病性毒株的特征,证明为低致病性毒株。其HA推导后氨基酸序列与H5N1AIV的同源性接近90%,以其研究的疫苗,可以有效抵御我国流行的H5亚型AIV病毒的感染,同时因为是弱毒株,以其研制的疫苗具有更好的安全性,也更符合公共卫生学的要求。
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| 关 键 词： | 禽流感病毒 H_5N_2亚型 HA基因 序列分析 |
| 文章编号： | 1005-944X(2006)10-0019-04 |
Cloning and Sequence Analysis of Hemagglutin in Gene of Avian Influenza Virus N/Ostrick/Denmark/72420/96(LPAIV-H5N2) |
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| Li Mingyi,Yu Shutao,Liu Xinwen,Gong Xiao,Guo Yuguang,Sui Guoning.Cloning and Sequence Analysis of Hemagglutin in Gene of Avian Influenza Virus N/Ostrick/Denmark/72420/96(LPAIV-H5N2)[J].China Journal Of Animal Quarantine,2006,23(10):19-22. |
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| Authors: | Li Mingyi Yu Shutao Liu Xinwen Gong Xiao Guo Yuguang Sui Guoning |
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| Abstract: | In order to analyze sequence similarities of hemagglutinin(HA) gene among avian influenza virus(AIV) subtype H5N2 isolates, the HA gene of avian influenza virus A/Ostrich/Denmark/72420/96 (LPAI-H5N1) isolate was amplified by RT-PCR according to the sequence published on GenBank.Then, the sequences were compared with GenBank data. The result showed that the cDNA contained 1737bp covering the whole ORF of HA gene. The HA gene sequence of the isolated strain shared about 80% nudleotide sequence identity with H5N1 isolates, but about 97% with some H5N2 isolates.The results confirmed the high frequence of recombination of the eight segment of AIV genome and the high variation ability of AIV. The amino acids of the cleavage site were E----T-R, confirmed that LPAI-H5N1 was a low pathogenic strain. The amino acid sequence shared about 90% identity with H5N1 isolates and the antigenicity was almost the same. So the H5N2 vaccine can protect the chickens from H5 subtype infection. |
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| Keywords: | Avian Influenza Virus Subtype H_5N_2 HA gene Sequence Analysis |
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