| 紫外分光光度法测定大豆中异黄酮的含量 |
| |
| 引用本文： | 黄芸,崔力剑,窦玉红,王鑫国,牛丽颖.紫外分光光度法测定大豆中异黄酮的含量[J].大豆科学,2007,26(2):273-275. |
| |
| 作者姓名： | 黄芸 崔力剑 窦玉红 王鑫国 牛丽颖 |
| |
| 作者单位： | 河北医科大学生药学教研室,石家庄,050017;河北医科大学中医学院,石家庄,050091 |
| |
| 基金项目： | 河北省科学技术研究与发展计划 |
| |
| 摘 要： | 建立一种测定大豆中异黄酮类成分含量的紫外分光光度法分析方法.以染料木素为对照品,利用染料木素与氢氧化钠产生反应,在271 nm波长处有最大吸收峰,用紫外分光光度法测定大豆中总异黄酮的含量.大豆中总异黄酮的含量为4.784 mg/g,加样回收率为100.9%,相对标准偏差为2.0%.方法简便、准确、重现性好,可作为检查大豆中异黄酮的含量的一种手段,适用于大豆及其保健品的日常分析和质量控制.
|
| 关 键 词： | 大豆异黄酮 氢氧化钠 分光光度法 含量测定 |
| 文章编号： | 1000-9841(2007)02-0273-03 |
| 修稿时间： | 2006年9月4日 |
DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY |
| |
| HUANG Yun,CUI Li-jian,DOU Yu-hong,WANG Xin-guo,NIU Li-ying.DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY[J].Soybean Science,2007,26(2):273-275. |
| |
| Authors: | HUANG Yun CUI Li-jian DOU Yu-hong WANG Xin-guo NIU Li-ying |
| |
| Abstract: | A UV spectrophotometry used for determination of soybean isoflavones was established.Genistein was selected as the reference substance.Based on the reaction of genistein and NaOH,which has the maximum absorption in 271 nm wavelength,ultra-violet spectrophotometry method was used for determination of isoflavones in Glycine max(L.) Merr..The total content of isoflavones in soybean extracts was 4.784 mg/g with mean recovery 100.9%,and the RSD was 2.0%.The method is suitable for the routine analysis and quality control of the soybean and function food with easy-to-handle,good accuracy and reproducibility. |
| |
| Keywords: | Soybean isoflavones NaOH UV spectrophtometry Quantitative determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
