| 基于LAMP方法的苜蓿疫霉根腐病菌分子检测研究 |
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| 引用本文： | 张裕君,刘跃庭,廖芳,罗加凤,黄国明.基于LAMP方法的苜蓿疫霉根腐病菌分子检测研究[J].中国植保导刊,2011,31(10). |
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| 作者姓名： | 张裕君 刘跃庭 廖芳 罗加凤 黄国明 |
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| 作者单位： | 天津出入境检验检疫局,天津,300461 |
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| 基金项目： | 天津出入境检验检疫局科技计划项目(TK030-2009) |
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| 摘 要： | 苜蓿疫霉根腐病菌是我国检疫性有害生物之一.但是,目前国内尚无检测标准.笔者针对该病菌特异分子标记,设计了LAMP引物,建立了苜蓿疫霉根腐病菌恒温快速检测方法(巳申请发明专利).该方法快速、准确、无需PCR仪,很好地满足了口岸检疫部门的检测要求.
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| 关 键 词： | 苜蓿疫霉根腐病菌 环介导 等温扩增 |
Research on molecular detection of Phytophthora medicaginis based on LAMP |
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| ZHANG Yu-jun,LIU Yue-ting,LIAO Fang,LUO Jia-feng,HUANG Guo-ming.Research on molecular detection of Phytophthora medicaginis based on LAMP[J].China Plant Protection,2011,31(10). |
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| Authors: | ZHANG Yu-jun LIU Yue-ting LIAO Fang LUO Jia-feng HUANG Guo-ming |
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| Institution: | ZHANG Yu-jun,LIU Yue-ting,LIAO Fang,LUO Jia-feng,HUANG Guo-ming(Tianjin Entry-Exit Inspection and Quarantine Bureau,Tianjin 300461,China) |
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| Abstract: | Phytophthora medicaginis was one of the quarantine pests in China,and there was no standard on its detection at present.Isothermal amplification method was established to detect the pathogen based on special LAMP primers designed by specific molecular mark in P.medicaginis,which will meet the port quarantine requirement with its characteristics of nicety,quickness and no PCR machine required. |
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| Keywords: | Phytophthora medicaginis loop-mediated isothermal amplification |
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