| 大丽轮枝菌拮抗细菌枯草芽孢杆菌8-28菌株产芽孢条件优化 |
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| 引用本文： | 王树香,王伟,李术娜,王全,李红亚,朱宝成.大丽轮枝菌拮抗细菌枯草芽孢杆菌8-28菌株产芽孢条件优化[J].湖北农业科学,2009,48(6). |
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| 作者姓名： | 王树香 王伟 李术娜 王全 李红亚 朱宝成 |
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| 作者单位： | 河北农业大学生命科学学院,河北,保定,071001 |
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| 摘 要： | 为提高大丽轮枝菌(Verticillium dahilae Kleb)拮抗细菌枯草芽孢杆菌(Bacillus subtilis)8-28菌株的芽孢形成率及芽孢数量,在摇瓶发酵基础上对影响菌株芽孢形成主要因素进行研究.通过单因素试验确定最佳碳源麦芽糊精、氮源黄豆饼粉、无机盐K2HPO4和MuSO4·H2O;采用正交试验对发酵培养基进行优化,摇瓶发酵生产芽孢的最佳条件为:麦芽糊精2.0%、黄豆饼粉2.0%、K2HPO40.05%和MnSO4·H2O0.01%、培养液初始pH值为7.0、种龄18 h、装瓶量50mL·(250mL)-1.在此优化条件下,发酵液中8-28茸株的芽孢微量迭到2.67×1 08个·mL-1.芽孢形成率迭到90%.
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| 关 键 词： | 大丽轮枝菌 枯草芽孢杆菌 发酵 芽孢 |
Optimization of Spore Production Conditions of Antagonistic Strain 8-28 of Bacillus subtilis against Verticillium dahilae |
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| WANG Shu-xiang,WANG Wei,LI Shu-na,WANG Quan,LI Hong-ya,ZHU Bao-cheng.Optimization of Spore Production Conditions of Antagonistic Strain 8-28 of Bacillus subtilis against Verticillium dahilae[J].Hubei Agricultural Sciences,2009,48(6). |
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| Authors: | WANG Shu-xiang WANG Wei LI Shu-na WANG Quan LI Hong-ya ZHU Bao-cheng |
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| Institution: | College of Life Sciences;Hebei Agricultural University;Baoding 071001;Hebei;China |
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| Abstract: | In order to improve the spore production rate and spore quantity of antagonistic strain 8-28 against Verticillium dahliae,the main factors influencing spore production was in vestigated by using shake-flask fermentation method.Through single factor experiment and orthogonal experiment,the optimal fermentation condition of strain 8-28 was determined as fol-lows: media composed of maltodextrin 2.0%,soybean meal 2.0%,KH2PO4 0.05% and MnSO4.H2O 0.01%,initial pH 7.0,culture time 18 h and bottle filling capacity ... |
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| Keywords: | Verticillium dahilae Bacillus subtilis fermentation spore |
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