| 我国1株鹦鹉幼稚病毒的细胞培养特性 |
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| 引用本文： | 李天宪,冯锋,夏苇,陈绳亮,赵林.我国1株鹦鹉幼稚病毒的细胞培养特性[J].中国兽医学报,2000,20(2):138-140. |
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| 作者姓名： | 李天宪 冯锋 夏苇 陈绳亮 赵林 |
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| 作者单位： | 中国科学院,武汉病毒研究所,湖北,武汉,430071 |
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| 基金项目： | 1997年湖北省重点科技项目! ( 971P0 80 6) |
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| 摘 要： | 为进一步研究鹦鹉幼稚病病毒（ＢＦＤＶ）的分子生物学特性奠定基础，对ＢＦＤＶ湖北分离株进行了２种原代细胞的适应性培养。首先在鹦鹉胚成纤维细胞盲传至第５代后再过渡到鸡胚成纤维细胞培养，建立了ＢＦＤＶ的适应细胞培养体系。观察了３种不同温度对细胞病变（ＣＰＥ）的影响，结果表明，ＢＦＤＶ在３８．５℃培养４８～９６ｈ可产生明显的ＣＰＥ。再将该培养液做免疫扩散试验结果出现明显的免疫复合物沉淀线，抗体中和试验显示
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| 关 键 词： | 鹦鹉幼稚病病毒 鸡胚成纤维细胞 细胞病变 |
| 修稿时间： | 1999-03-10 |
Growth Characteristics of a New Strain of Psittacine Virus Found in China |
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| LI Tian-xian,FENG Feng,XIA Wei,CHEN Sheng-liang,ZHAO Lin.Growth Characteristics of a New Strain of Psittacine Virus Found in China[J].Chinese Journal of Veterinary Science,2000,20(2):138-140. |
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| Authors: | LI Tian-xian FENG Feng XIA Wei CHEN Sheng-liang ZHAO Lin |
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| Abstract: | A
new strain of psittacine virus was isolated fro m fledgli ng budgerigars by our laberatory
in 1995, and had been designated as Budgerigar fledgling disease virus(BFDV). The virus
was cultured initially on budgerigar em bryo fibroblast(BEF) for 5 generations, then
cultured on chicken embryo fibroblast (CEF) for 13 generations. The tissue culture of BFDV
showed the cytopathogenic effect (CPE ) under 38.5℃ for 48-96 h. |
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| Keywords: | Budgerigar fledgling disease virus cytopathogenic effect chicken embryo fibroblast |
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