The use of allozymes at three life stages influences genetic variation analysis and clustering in Cicer arietinum germplasm collections |
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| Authors: | L Triest SMG Kabir |
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| Institution: | (1) Laboratory for General Botany and Nature Management, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium |
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| Abstract: | Levels of genetic variation using 6 enzyme systems for a total of 11 interpretable loci were examined in chickpea (Cicer arietinum) originating from 9locations in Bangladesh. The measurement of genetic variation at enzyme loci was carried out on the seed
embryo, on the early leaves of seedlings and on the mature leaves at the vegetative stage. A total of 592individuals, including
240 seeds, 200 seedlings and152 mature leaves were investigated. Using electrophoretic data, chickpea was found to express
higher percentages of polymorphic loci at the seed stage (36–64%) than at seedling (22–56%) or the vegetative stage (11–44%).
The proportion of mean number of alleles and the average mean observed heterozygosity also were higher at the seed stage when
compared to the seedling and vegetative stages. Unique alleles were absent, and only differences infrequencies could be noticed.
Positive values of the fixation index were noted for pgm-1 and 6pg-1 for all stages and in both mnr loci for the seed embryo's.
A trend towards lower genetic distances of all possible pairs of populations could be observed when comparing those of seed
embryo's with seedlings or mature leaves. This trend was even more pronounced when pooling the data of 9 populations into
their 3regions. Slight differences in genetic distances caused a separative clustering of population 3 at seed embryo, of
population 2 at seedling and of population5 at vegetative stages. It is suggested that careful examination of enzyme polymorphisms
at different developmental stages is a prerequisite before drawing conclusions on the genetic distance between germplasm collections
from different origins since small differences in the data entry for clustering results in ties that may affect tree topologies.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | allozymes Bangladesh chickpea Cicer arietinum conservation genetics germplasm populations variation |
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