| 基于COI基因探讨塔里木河流域裂腹鱼属鱼类的亲缘关系 |
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| 引用本文： | 海萨·艾也力汗,张人铭,牛建功,胡江伟,刘鸿,张钰,吐尔逊·提立瓦尔地,封永辉.基于COI基因探讨塔里木河流域裂腹鱼属鱼类的亲缘关系[J].中国水产科学,2021,28(11):1417-1425. |
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| 作者姓名： | 海萨·艾也力汗 张人铭 牛建功 胡江伟 刘鸿 张钰 吐尔逊·提立瓦尔地 封永辉 |
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| 作者单位： | 新疆维吾尔自治区水产科学研究所, 新疆 乌鲁木齐 830000 |
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| 基金项目： | 农业农村部“西北地区重点水域渔业资源与环境调查”专项. |
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| 摘 要： | 裂腹鱼属(Schizothorax)是塔里木河流域分布的裂腹鱼亚科(Schizothoracids)鱼类中数量和种类最多的鱼类, 具有重要的生态学研究价值。本研究对采自塔里木河和伊犁河流域的 6 种裂腹鱼属鱼类的 COI 基因序列进行了分析。结果显示, 在 419 尾个体中共发现 25 个单倍型; 塔里木裂腹鱼(Schizothorax biddulphi)塔里木河主要支流群体、 宽口裂腹鱼(S. eurystomus)、重唇裂腹鱼(S. barbatus)和厚唇裂腹鱼(S. irregularis)之间的遗传距离小于 0.005, 未形成明显的“条形码间隙”。而塔里木裂腹鱼车尔臣河群体与其他物种间的遗传距离符合 2%阈值或 10 倍原则; ABGD 和 TaxonDNA 分析结果相同, 把新疆裂腹鱼属鱼类划分为 4 个组别, 塔里木裂腹鱼塔里木河主要支流群体、宽口裂腹鱼、重唇裂腹鱼和厚唇裂腹鱼分为一组, 伊犁裂腹鱼(S. pseudaksaiensis)、银色裂腹鱼(S. argentatus)、塔里木裂腹鱼车尔臣河群体分别划分为另外 3 个组别中; 贝叶斯法(BI)系统发育树中塔里木裂腹鱼车尔臣河群体形成了独立的分支, 节点支持率为 100%, 而塔里木裂腹鱼塔里木河主要支流群体、宽口裂腹鱼、重唇裂腹鱼和厚唇裂腹鱼未能形成独立的分支。BI 树拓扑结构与 ABGD 和 TaxonDNA 分组结果一致。塔里木裂腹鱼塔里木河主要支流群体、宽口裂腹鱼、重唇裂腹鱼和厚唇裂腹鱼可能存在种间杂交或尚未达到物种分化程度, 而塔里木裂腹鱼车尔臣河群体可能已分化成独立的物种。
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| 关 键 词： | 塔里木河 裂腹鱼属 DNA 条形码 遗传距离 |
Relationship of Schizothorax Heckel in Tarim River basin based on COI gene |
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| HAYSA Ayelhan,ZHANG Renming,NIU Jiangong,HU Jiangwei,LIU Hong,ZHANG Yu,TURSUN Tiliwaerdi,FENG Yonghui.Relationship of Schizothorax Heckel in Tarim River basin based on COI gene[J].Journal of Fishery Sciences of China,2021,28(11):1417-1425. |
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| Authors: | HAYSA Ayelhan ZHANG Renming NIU Jiangong HU Jiangwei LIU Hong ZHANG Yu TURSUN Tiliwaerdi FENG Yonghui |
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| Institution: | Xinjiang Fishery Research Institute, Urumqi 830000 , China |
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| Abstract: | Tarim River is the largest one of the inland rivers in China. The genus Schizothorax Heckel has the most abundant and diverse fish in the subfamily of Schizothoracinae distributed in the Tarim River Basin and has a great ecological value of researches. In this study, the phylogenetic relationships of 6 species of fish in genus Schizothorax Heckel collected from Tarim River basin and Yili River basin were analyzed using the mitochondrial COI gene sequences. A total of 25 haplotypes were detected from 419 individuals of samples. The genetic distances among Gunther population of S. biclolulphi collected from the Tarim River main tributary, S. eurystomus (Kessler), S. barbatus McClelland, and S. irregularis Day were all less than 0.005, and no significant "barcode gap" was formed. However, the genetic distances between the Qarqan River population of S. biclolulphi Gunther and other species were accorded with the 2% threshold or the principle of ten times. Both of the ABGD and Taxon DNA analysis identically showed that the Xinjiang Schizothorax Heckel were divided into four groups. Gunther population of S. biclolulphi from Tarim River main tributary, S. eurystomus, S. barbatus and S. irregularis were formed one group. S. pseudaksaiensis, S. argentatus, and S. biclolulphi population from Qarqan River were formed the other three groups, respectively. In the BI phylogenetic tree, the S. biclolulphi Qarqan River population formed an independent branch with a node support rate with 100%. However, S. biclolulphi population from Tarim River main tributary, S. eurystomus, S. barbatus, and S. irregularis failed to form independent branches. The topology of BI tree is consistent with the grouping results of ABGD and Taxon DNA. Interspecific hybridization may be exists among S. biclolulphi population from Tarim River main tributary, S. eurystomus, S. barbatus, and S.irregularis, or these species has not yet reach the interspecific differentiation degree. S. biclolulphi population from Tarim River may have diverged into separate species. These results will provide a reference for analyze the genetic relationship of fish in genus Schizothorax Heckel from Tarim River Basin. |
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