| 福建省樟树溃疡病病原菌的分离与鉴定 |
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| 作者姓名： | 张晓阳 吴松 王美鑫 韩森汇 宋漳 陈全助 |
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| 作者单位： | 福建农林大学林学院;福建农林大学金山学院 |
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| 基金项目： | 福建省科技厅项目(K1518090A,K1518075A);福建农林大学科技创新专项(CXZX2017121,CXZX2017501)。 |
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| 摘 要： | 为了明确福建省樟树溃疡病的病原菌种类,对福建省福州市仓山区、三明市明溪县、南平市建阳区和顺昌县4个地区采集的樟树枝干溃疡样本进行分离、纯化和致病性分析,将病原菌进行系统形态学观察,并结合代表菌株的核糖体基因内转录间隔区(ITS)、翻译延伸因子(EF-1α)、RNA聚合酶Ⅱ大亚基(RPB2)和β-微管蛋白(BT)基因序列进行同源性分析,构建进化树,最终确定病原菌种类。结果表明,在福建省不同地区内采集到的75份樟树溃疡样本,经过组织分离、纯化和致病性检测,得到43个病原菌株,根据观察病原菌的菌丝生长状态和孢子特征,并结合系统进化树的结果,得到小新壳梭孢(Neofusicoccum parvum)、可可毛色二孢(Lasiodiplodia theobromae)、假可可毛色二孢(Lasiodiplodia pseudotheobromae)和Lasiodiplodia iranensis 4种类型菌株。接种菌株后发病症状与野外发病症状相同,但致病程度不同,其中小新壳梭孢最强,可可毛色二孢次之,L.iranensis最弱。
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| 关 键 词： | 樟树 溃疡病 病原鉴定 毛色二孢属 新壳梭孢属 致病性 福建省 |
Isolation and identification of camphor tree canker disease pathogen in Fujian Province |
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| Authors: | ZHANG Xiaoyang WU Song WANG Meixin HAN Senhui SONG Zhang CHEN Quanzhu |
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| Institution: | (College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Jinshan College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | This study aimed to identify the Cinnamomum camphora canker disease pathogen in Fujian Province.We isolated,purified,and analyzed the pathogenicity of canker samples from four districts including Fuzhou City,Mingxi County of SanmingCity,Jianyang District,and Shunchang County of Nanping City to determine the pathogen.The morphological characteristics of the pathogens were combined with the ITS,EF-1α,RPB2,and BT gene sequences of the representative strains for amplification and sequencing.This allowed the construction of the evolutionary tree,and the pathogens were finally determined.Among 75 dry ulcer samples collected from the camphor trees in different regions of Fujian Province,43 pathogenic strains were obtained after tissue isolation,purification,and pathogenicity testing.Based on the status of mycelium growth and spore characteristics of pathogenicfungi and the results of phylogenetic trees,four strains were obtained,namely:Neofusicoccum parvum,Lasiodiplodia theobromae,Lasiodiplodia pseudotheobromae,and Lasiodiplodia iranensis.The pathogenicity test results showed that N.parvum was the strongest pathogen,followed by L.theobromae,and L.iranensis was the weakest. |
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| Keywords: | Cinnamomum camphora canker disease pathogen identification Lasiodiplodia Neofusicoccum pathogenicity Fujian Province |
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