| 45S rDNA在7种竹子植物染色体上的定位 |
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| 引用本文： | 徐川梅,卢江杰,汤定钦.45S rDNA在7种竹子植物染色体上的定位[J].林业科学,2009,45(12). |
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| 作者姓名： | 徐川梅 卢江杰 汤定钦 |
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| 作者单位： | 浙江林学院,浙江省现代森林培育技术重点实验室,临安,311300 |
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| 基金项目： | 国家自然科学基金项目，浙江省自然科学基金人才项目 |
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| 摘 要： | 编码18-5.8-25S核糖体RNA的45S rDNA基因,是1个简单的多基因家族基因,一般以串联方式相连,对应于核仁组织区(NOR).首次利用荧光原位杂交技术(FISH),研究45S rDNA在散生竹类的毛竹和斑竹,混生竹类的茶秆竹、日本矮竹、菲白竹和铺地竹,以及丛生竹类的白绿竹染色体上的分布.结果表明:本研究所涉及的散生竹和混生竹的几个竹种,只观察到1对随体染色体的次缢痕区域有45S rDNA位点,而丛生竹类的白绿竹除随体染色体具有45S rDNA位点外,某些非随体染色体上也有不同拷贝数的45S rDNA位点存在.
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| 关 键 词： | 次缢痕 竹子 |
Physical Mapping of the 45S rDNA on Metaphase Chromosomes in Seven Bamboo Species by in situ Hybridization |
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| Xu Chuanmei,Lu Jiangjie,Tang Dingqin.Physical Mapping of the 45S rDNA on Metaphase Chromosomes in Seven Bamboo Species by in situ Hybridization[J].Scientia Silvae Sinicae,2009,45(12). |
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| Authors: | Xu Chuanmei Lu Jiangjie Tang Dingqin |
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| Abstract: | Fluorescence ire situ hybridization ( FISH) was first applied in locating 45S rDNA sequence on metaphase chromosomes of Phyllostachys edulis, Ph . bambusoides f. lacrima-deae, Pseudosasa amabilis , Sasa fortunei, S . pygmea, S .argenteastriatus and Bambusa oldhamii. The 45S rDNA locus was detected in the secondary chromosomal constriction region for all the bamboo species investigated. Additional 45S rDNA loci were also found in non-satellite chromosomes in a sympodial bamboo species-B . oldhamii. |
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| Keywords: | FISH 45SrDNA FISH 45S rDNA secondary constriction regions bamboo |
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