| 亚临界R134a体系中的绿色酶促反应研究(英文) |
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| 引用本文： | 张慧,于刚,王玉明,薛长湖.亚临界R134a体系中的绿色酶促反应研究(英文)[J].广东农业科学,2011,38(13). |
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| 作者姓名： | 张慧 于刚 王玉明 薛长湖 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,广东,广州,510300
2. 中国海洋大学食品科学与工程学院,山东,青岛,266003 |
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| 基金项目： | 国家自然科学基金(30972285); 广东省海洋渔业科技推广专项(A200901Ⅰ03); 中央级科研院所基本科研业务费专项(2010YD11) |
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| 摘 要： | 以环境友好的亚临界1,1,1,2-四氟乙烷(R134a)作为反应介质,用磷脂酶D催化卵磷脂(PC)的酰基转移反应制备磷脂酰丝氨酸(PS)。探讨了压力、温度、水分、酶量、底物对酶促反应的影响。结果表明,磷脂酶D在亚临界R134a中(303～323 K、2～8MPa)表现出较高的催化活性。与传统的有机溶剂反应体系相比,亚临界R134a体系能够改善酶促反应动力学,即需要较少的酶量即可迅速达到较高的转化率;在亚临界R134a体系压力6 MPa、体系温度313 K的条件下,9.6 U磷脂酶D催化反应240 min,磷脂酰丝氨酸的含量达到84.88%。
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| 关 键 词： | 酰基转移 磷脂酶D 活性 亚临界R134a |
Green enzymatic process in subcritical R134a |
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| ZHANG Hui,YU Gang,WANG Yu-ming,XUE Chang-hu.Green enzymatic process in subcritical R134a[J].Guangdong Agricultural Sciences,2011,38(13). |
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| Authors: | ZHANG Hui YU Gang WANG Yu-ming XUE Chang-hu |
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| Institution: | ZHANG Hui1,YU Gang1,WANG Yu-ming2,XUE Chang-hu2(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China,2.Department of Food Science and Engineering,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | Environmentally benign subcritical 1,1,1,2-tetrafluoroethane (R134a) was successfully used as a medium for enzymecatalyzed synthesis.Phospholipase D -mediated transphosphatidylation to phosphatidylserine (PS) from phosphatidylcholine (PC) was performed in subcritical R134a and the influence of several parameters (enzyme loading, pressure, temperature, water addition and substrates) on the conversion was investigated. The results indicated that phospholipase D was active in subcritical R134a at 303～323 K and 2～8 MPa. Subcritical R134a reaction mixture resulted in enhancing the reaction kinetics with respect to conventional organic solvent system, requiring lower enzyme concentrations desired at commercial industrial scales. Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum PS content of 84.88% was attained at 6 MPa, 313 K, after 240 minutes with 9.6 units phospholipase D. |
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| Keywords: | transphosphatidylation phospholipase D active subcritical R134a |
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