| 一种新的非酶法分离荠幼胚的方法 |
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| 引用本文： | 洪亚平,张亚冰,吴国锋,赵文娟,阎婵婵.一种新的非酶法分离荠幼胚的方法[J].安徽农业科学,2009,37(23):10852-10853. |
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| 作者姓名： | 洪亚平 张亚冰 吴国锋 赵文娟 阎婵婵 |
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| 作者单位： | 河南科技大学农学院,河南洛阳,471003 |
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| 基金项目： | 国家自然科学基金，河南科技大学SRTP项目 |
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| 摘 要： | 目的]用非酶法手工分离荠的胚珠和幼胚等微小结构。方法]应用胶块和解剖镜等普通工具，手工、非酶法分离并观察荠的花形态及荠胚珠和幼胚。结果]荠花具有4片花萼、4片花瓣、四强雄蕊，短角果；荠的胚发育过程包括球形胚、心形胚和鱼雷形胚3个发育阶段。结论]利用胶块的黏性，能使植物胚珠和胚等微小结构的手工分离操作变得比较简单，具有对幼胚损伤小、能保持原有形态等优点。可用于多领域的研究。该类工作在实验设施较差的实验室也能完成。
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| 关 键 词： | 荠 胚珠 胚 胶块 分离 |
A New Method to Non-enzymatically Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic. |
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| HONG Ya-ping et al.A New Method to Non-enzymatically Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic.[J].Journal of Anhui Agricultural Sciences,2009,37(23):10852-10853. |
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| Authors: | HONG Ya-ping |
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| Institution: | HONG Ya-ping et al(College of Agronomy,Henan University of Science , Technology,Luoyang,Henan 471003) |
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| Abstract: | Objective] The ovules and immature embryos were manually isolated by non-enzymatic method.Method] The ordinary tools such as rubber block and anatomical lens were used to isolate ovule and immature embryo as well as observe floral characteristics.Result] C.bursa-pastoris.had four calyxes,four petals and tetradynamous stamen,while the development process of embryo of C.bursa-pastoris.included three developmental stages,namely,globular embryo,heart-shape embryo and torpedo-shaped embryo.Conclusion]Using t... |
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| Keywords: | Capsella bursa-pastoris(L ) Medic Ovule Embryo Gum block Isolation |
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