| 番鸭呼肠孤病毒感染对体液免疫功能的影响 |
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| 引用本文： | 王全溪,吴宝成,李国平,黄一帆.番鸭呼肠孤病毒感染对体液免疫功能的影响[J].中国农业科学,2010,43(2):424-429. |
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| 作者姓名： | 王全溪 吴宝成 李国平 黄一帆 |
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| 作者单位： | （福建农林大学动物科学学院） |
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| 摘 要： | 【目的】探讨番鸭呼肠孤病毒感染对体液免疫功能的影响。【方法】将60只5日龄健康番鸭,随机平均分为两组,分室隔离饲养。试验组雏鸭每只腿部肌肉注射番鸭呼肠孤病毒细胞毒0.2 mL（0.01个TCID50=10-3.7558）,对照组相同部位注射等量灭菌生理盐水,每隔5 d,应用组织化学法检测脾脏和法式囊中浆细胞数量,间接血凝法检测血清中禽流感抗体水平和放射免疫法检测血清中IL-2,IL-6含量的变化。【结果】番鸭感染呼肠孤病毒,法氏囊和脾脏中浆细胞数量试验组均比对照组少,在感染后15 d达到最少,试验组极显著地低于对照组（P＜0.01）;同时,番鸭感染呼肠孤病毒可抑制禽流感抗体形成,感染后15d抗体水平最低,试验组极显著地低于对照组（P＜0.01）,此后又逐渐升高;血清中IL-2和IL-6的含量也在感染初期降低后又逐渐回升,感染后10 d均达最低,试验组极显著地低于对照组（P＜0.01）。【结论】番鸭呼肠孤病毒感染5日龄雏番鸭,通过破坏免疫器官(法氏囊,脾脏)中的浆细胞,降低了机体抗体形成能力,同时,也通过对免疫分子的影响反过来影响机体的体液免疫功能。提示,增强机体体液免疫功能是防制该病的一个途径。
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| 关 键 词： | 番鸭呼肠孤病毒 体液免疫 浆细胞 IL-2 IL-6 |
| 收稿时间： | 2008-10-21; |
Effects of Duck Infected by Muscovy Duck Reovirus on Humoral Immunity Function |
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| WANG Quan-xi,WU Bao-cheng,LI Guo-ping,HUANG Yi-fan.Effects of Duck Infected by Muscovy Duck Reovirus on Humoral Immunity Function[J].Scientia Agricultura Sinica,2010,43(2):424-429. |
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| Authors: | WANG Quan-xi WU Bao-cheng LI Guo-ping HUANG Yi-fan |
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| Institution: | (College of Animal Science, Fujian Agriculture and Forestry University) |
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| Abstract: | Objective] The purpose of the test was to investigate the effect of muscovy duck infected by muscovy duck reovirus on humoral immunity. Method] Sixty healthy ducks at 5-day-old of age were randomly divided into two groups . The experimental group was injected 0.2ml muscovy duck reovirus (one penent of TCID_(50), TCID_(50)=10~(3.7558)) on leg, and the controll group was injected normal saline at the same quantity. The quantity of plasma cells in bursa fabricius and the spleen was tested by the histochemical test method. The level of avian influenza antibodies in serum was tested by the method of indirect hemagglutination, also the content change of EL-2 and IL-6 in the serum was tested by the testing method of radiation immunization. Result] Results showed that the quantity of plasma cells in bursa of fabricius and spleen in the experiment group was less than the controll group, had different degrees of decrease respectively during the time after attack test by using reovirus, especially after 15 day of infection the antibody of experiment group was the lowest (P<0.01). The ability of antibody-producing decreased too. The avian influence antibody of experiment group was very significantly less than the contrail group (P<0.01), especially after 15 day of infection the antibody of experiment group was the lowest. But they all increased at 15 days after attack test .The content of EL-2 and IL-6 in the serum of experiment group ducks decreased on the first stage of the test ,but they all increased on the later stage compared with the former. However the content of IL-2 and IL-6 in the serum of experiment group ducks was lower than the control group (P<0.01). Conclusion] Ducks at five day old of age are infected by muscovy duck reovirus, plasma cells in immune organs (bursa of fabricius and spleen) are damaged and the ability of antibody forming decrease. The immunity function of ducks decrease because the virus decreased the content of immunity molecular (IL-2, IL-6) in serum. Therefore it is an effective way to enhance the humoral immunity of duck to control muscovy duck reovirus. |
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| Keywords: | IL-2 IL-6 muscovy duck reovirus humoral immunity plasma cell IL-2 IL-6 |
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