| 应用Taqman荧光定量PCR快速检测宠物食品中的沙门氏菌 |
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| 引用本文： | 贾俊涛,崔鹤,马云,曾静,姜英辉,李正义.应用Taqman荧光定量PCR快速检测宠物食品中的沙门氏菌[J].安徽农业科学,2012(30):14973-14975. |
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| 作者姓名： | 贾俊涛 崔鹤 马云 曾静 姜英辉 李正义 |
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| 作者单位： | 山东出入境检验检疫局检验检疫技术中心;北京出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 质检总局公益资助项目(201110034) |
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| 摘 要： | 目的]建立快速检测宠物食品中沙门氏菌(Salmonella)的荧光定量PCR方法。方法]根据Genbank中登录的沙门氏菌吸附和侵袭上皮细胞表面蛋白invA基因序列设计合成引物和探针,检测该方法的特异性和敏感性。结果]该方法具有良好的特异性,对沙门氏菌的检测灵敏度可达到17 CFU/反应(25μl/反应)。应用该方法检测人工污染宠物食品,样品经18 h增菌后,结果均为阳性。结论]该研究建立的Taqman荧光定量PCR方法可以快速、准确地检测宠物食品中的沙门氏菌。
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| 关 键 词： | 荧光定量PCR 沙门氏菌 Taqman探针 检测 |
Taqman PCR-based Methods for Rapid Detection of Salmonella in Pet Food |
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| Institution: | JIA Jun-tao et al(Technology Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao,Shandong 266002) |
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| Abstract: | Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food.Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica.Result] The assay detects Salmonella specifically.The detection limit of the real-time PCR was 17 CFU/test(25 μl/test) for the positive strain.This method was effective to detect artificially contaminated pet food.Conclusion] The results showed that Taqman PCR assay was rapid and accure for detection of Salmonella from infected pet food. |
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| Keywords: | Fluorescence quantitative PCR Salmonella Taqman probe Detection |
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