| 西北酸菜直投式发酵剂菌株高密度培养的研究 |
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| 引用本文： | 葛宗昌,孟宪刚.西北酸菜直投式发酵剂菌株高密度培养的研究[J].安徽农业科学,2012(27):13566-13569. |
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| 作者姓名： | 葛宗昌 孟宪刚 |
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| 作者单位： | 兰州交通大学化学与生物工程学院,甘肃兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术专项(GNSW2009-11);兰州交通大学青蓝人才项目(QL0515A) |
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| 摘 要： | 目的]制备出适合西北酸菜发酵的复合直投式发酵剂。方法]前期筛选出3个菌株:产酸速率快的东方醋酸杆菌AC5,降解亚硝酸盐高的干酪乳杆菌L5,醇香味适中的东方伊萨酵母Y7。对这3种菌分别进行高密度培养研究,确定菌体增殖的最佳培养基及培养条件。结果]确定增殖培养基的成分是马铃薯汁、番茄汁、葡萄糖、蛋白胨和磷酸盐。通过正交试验和培养条件优化得出,AC5的最佳培养基为马铃薯汁含量40 g/L、番茄汁含量80 g/L、葡萄糖含量20 g/L、胰蛋白胨含量10 g/L、磷酸盐含量10 g/L,在pH 5.5、30℃、150r/min下培养24 h活菌数达到3.42×108cfu/ml;L5的最佳培养基为马铃薯汁含量40 g/L、番茄汁含量60 g/L、葡萄糖含量5 g/L、胰蛋白胨含量15 g/L、磷酸盐含量10 g/L,在pH 6.5、30℃、120 r/min下培养24 h活菌数可达到1.93×109cfu/ml;Y7的最佳培养基为马铃薯汁含量60 g/L、番茄汁含量60 g/L、葡萄糖含量20 g/L、胰蛋白胨含量20 g/L、磷酸盐含量10 g/L,在pH 6.0、35℃、150 r/min下培养24h活菌数可达到2.74×108cfu/ml。结论]该研究使单位体积的活菌数有很大提高,为制备西北酸菜直投式发酵剂提供了依据。
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| 关 键 词： | 西北酸菜 直投式发酵剂 高密度培养 |
Study on High-density Cultivation of Directed Vat Set(DVS) for Fermented Vegetables in Northwest China |
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| Institution: | GE Zong-chang et al(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou,Gansu 730070) |
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| Abstract: | Objective] To prepare a compound DVS for fermented vegetables in Northwest China.Method] Three strains were affirmed: AC5(Acetobacter orientalis),which produces acid fast;L5(Lactobacillus casei),which degradates nitrite highly;Y7(Issatchenkia orientalis),which has rich mellow savour.Through the research on these three bacteria on the high density cultivation,the best medium and culture conditions of bacterial multiplication were determined.Result] The compositions of medium were potato juice,tomato juice,glucose,tryptone and phosphate.The results of orthogonal test indicated that suitable medium formulations of AC5 were 40 g/L potato juice,80 g/L tomato juice,20 g/L glucose,10 g/L tryptone and 10 g/L phosphate,the incubation temperature was 30 ℃,initial pH was 5.5,shaking frequence was 150 r/min.The cell population got to 3.42×108 cfu/ml after 24 hours culture.Suitable medium formulations of L5 were 40 g/L potato juice,60 g/L tomato juice,5 g/L glucose,15 g/L tryptone and 10 g/L phosphate,,incubation temperature was 30 ℃,initial pH was 6.5,shaking frequence was 120 r/min.The cell population got to 1.93×109 cfu/ml after 24 hours culture.Suitable medium formulations of Y7 were 60 g/L potato juice,60 g/L tomato juice,20 g/L glucose,20 g/L tryptone and 10 g/L phosphate,incubation temperature was 35 ℃,initial pH was 6.0,shaking frequence was 150 r/min.The cell population got to 2.74×108 cfu/ml after 24 hours culture.Conclusion] In this study,the per unit area number of viable organism has been greatly improved,providing basis for producing DVS of fermented vegetables in Northwest China. |
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| Keywords: | Fermented vegetables in Northwest China Directed vat set High-density cultivation |
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