| 贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量测定 |
| |
| 引用本文： | 张艳焱,徐剑,张永萍.贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量测定[J].安徽农业科学,2012(27):13322-13323. |
| |
| 作者姓名： | 张艳焱 徐剑 张永萍 |
| |
| 作者单位： | 贵阳中医学院药学院,贵州贵阳,550002 |
| |
| 基金项目： | 贵州省科技厅基金资助项目(黔科合中药专字200407号) |
| |
| 摘 要： | 目的]测定贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量。方法]采用反滴定法测定总生物碱含量,并采用高效液相色谱法测定白屈菜红碱的含量,色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm),流动相为乙腈-浓度0.1%磷酸水溶液(V/V,28∶72),检测波长为270 nm,流速为1.0 ml/min。结果]总生物碱、白屈菜红碱的平均回收率分别为97.5%和99.8%;RSD分别为2.71%和0.40%。结论]该分析方法准确、灵敏、可靠,可用于飞龙掌血中总生物碱和白屈菜红碱的含量测定。
|
| 关 键 词： | 飞龙掌血 总生物碱 白屈菜红碱 高效液相色谱法 |
Determination of Total Alkaloid and Chelerythrine in Toddalia Asiatica from Different Places in Guizhou |
| |
| Institution: | ZHANG Yan-yan et al(Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002) |
| |
| Abstract: | Objective] To determine the contents of total alkaloid and chelerythrine in Toddalia asiatica from different places in Guizhou.Method] Total alkaloid was determined by back-titrantion method,and chelerythrine was determined by HPLC method,using Diamonsil C18 column(250 mm×4.6 mm,5 μm),and acetonitrile-0.1% phosphoric acid(V/V,28∶72) as mobile phase,with the detection wavelength at 270 nm and flow rate of 1.0 ml/min.Result] The average recoveries of total alkaloid and chelerythrine were 97.5% and 99.8% with RSD = 2.71% and 0.40% respectively.Conclusion] This analysis method is accurate,sensitive and reliable to determine the contents of alkaloid in Toddalia asiatica. |
| |
| Keywords: | Toddalia asiatica Total alkaloid Chelerythrine HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
