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一株产蛋白酶地衣芽孢杆菌的分离与鉴定
引用本文:石笛,江洁芳,钟瑜,黄琼.一株产蛋白酶地衣芽孢杆菌的分离与鉴定[J].安徽农业科学,2012,40(29):14195-14196,14202.
作者姓名:石笛  江洁芳  钟瑜  黄琼
作者单位:广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663;广州市微生物研究所,广东广州,510663
摘    要:目的]对产蛋白酶的地衣芽孢杆菌进行分离与鉴定。方法]从饲料样品中筛选出产蛋白酶的菌株,对其进行形态和生理生化特征鉴定,并构建该菌株的16S rDNA系统发育树。结果]分离到的菌株GW201205与地衣芽孢杆菌(Bacillus licheniformis)的生理生化特征相同,且其16S rDNA序列与B.licheniformis相似性最高。结论]可将试验分离到的菌株GW201205初步鉴定为B.licheniformis。

关 键 词:蛋白酶  地衣芽孢杆菌  分离  鉴定

Isolation and Identification of a Bacillus licheniformis with Protease Activity
Institution:SHI Di et al(Guangzhou Microbe Research Institute,Guangzhou,Guangdong 510663)
Abstract:Objective] To isolate and identify a Bacillus licheniformis with protease activity.Method] A strain with protease activity was isolated from the feed samples,and then its morphological and biochemical characteristics were identified,a 16S rDNA phylogenetic tree of this strain was constructed.Result] The isolated strain GW201205 showed similar biochemical characteristics and 16S rDNA to Bacillus licheniformis.Conclusion] The isolated strain GW201205 is preliminarily identified to be Bacillus licheniformis.
Keywords:Protease  Bacillus licheniformis  Isolation  Identification
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