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两个鸢尾新品种组培苗生根培养的研究
引用本文:赵春莉,张芳,顾德峰,李金英.两个鸢尾新品种组培苗生根培养的研究[J].安徽农业科学,2012(27):13251-13253.
作者姓名:赵春莉  张芳  顾德峰  李金英
作者单位:吉林农业大学园艺学院,吉林长春,130118;吉林农业大学园艺学院,吉林长春,130118;吉林农业大学园艺学院,吉林长春,130118;吉林农业大学园艺学院,吉林长春,130118
基金项目:吉林省教育厅资助项目(吉教科合字〔2009〕第62号)
摘    要:目的]研究2个鸢尾新品种‘白天鹅’和‘黑色旗帜’的组培苗生根培养方法。方法]以2个德国鸢尾品种‘白天鹅’和‘黑色旗帜’组培苗的不定芽为外植体,研究不同培养基、NAA浓度及接种丛生芽数量对其生根培养的影响。结果]2个鸢尾新品种的最佳生根培养基为1/2MS+0.5 mg/L NAA;不定芽接种时其试管苗数量至少为3株/丛。结论]为2个鸢尾新品种‘白天鹅’和‘黑色旗帜’的快速繁殖提供了技术参考。

关 键 词:鸢尾  组织培养  生根

Study on the Rooting of Tissue Culture Seedlings from Two New Varieties of Iridaceae
Institution:ZHAO Chun-li et al(College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118)
Abstract:Objective] To study the rooting of the tissue culture seedlings from two new varieties of Iridaceae "White Swan" and "Black Flag".Method] With the adventitious buds from the two new varieties as explants,the effects of different media,NAA concentration,and inoculated buds number on the rooting culture were studied.Result] The best rooting culture medium for the two varieties was 1/2MS + 0.5 mg/L NAA.There should be at least three plants each cluster for the inoculation of adventitious buds.Conclusion] The study provides technical support for the rapid propagation of the two new Iridaceae varieties.
Keywords:Iridaceae  Tissue culture  Rooting
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